Interaction of myogenic factors and the retinoblastoma protein mediates muscle cell commitment and differentiation

Wei Gu, Jay W. Schneider, Gianluigi Condorelli, Sunjay Kaushal, Vijak Mahdavi, Bernardo Nadal-Ginard

Research output: Contribution to journalArticle

614 Scopus citations

Abstract

The experiments reported here document that the tumor suppressor retinoblastoma protein (pRB) plays an important role in the production and maintenance of the terminally differentiated phenotype of muscle cells. We show that pRB inactivation, through either phosphorylation, binding to T antigen, or genetic alteration, inhibits myogenesis. Moreover, inactivation of pRB in terminally differentiated cells allows them to reenter the cell cycle. In addition to its involvement in the myogenic activities of MyoD, pRB is also required for the cell growth-inhibitory activity of this myogenic factor. We also show that pRB and MyoD directly bind to each other, both in vivo and in vitro, through a region that involves the pocket and the basic-helix-loop-helix domains, respectively. All the results obtained are consistent with the proposal that the effects of MyoD on the cell cycle and of pRB on the myogenic pathway result from the direct binding of the two molecules.

Original languageEnglish (US)
Pages (from-to)309-324
Number of pages16
JournalCell
Volume72
Issue number3
DOIs
Publication statusPublished - Feb 12 1993

    Fingerprint

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Gu, W., Schneider, J. W., Condorelli, G., Kaushal, S., Mahdavi, V., & Nadal-Ginard, B. (1993). Interaction of myogenic factors and the retinoblastoma protein mediates muscle cell commitment and differentiation. Cell, 72(3), 309-324. https://doi.org/10.1016/0092-8674(93)90110-C