Interaction of posttetanic potentiation and the catchlike property in mouse skeletal muscle

William Gittings, Jordan Bunda, James T. Stull, Rene Vandenboom

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Introduction: Posttetanic potentiation (PTP) and the catchlike property (CLP) enhance contractile function in skeletal muscle. We investigated the CLP during dynamic performance in mouse hindlimb muscles with (wild-type) and without (skMLCK-/-) the primary mechanism for PTP (myosin phosphorylation) (in vitro, 25°C). Methods: Extensor digitorum longus muscles of both genotypes were stimulated with constant frequency and catchlike trains (CFT and CLT), before and after a potentiating stimulus (PS). Results: Before the PS, the CLT increased concentric force/work relative to the CFT, but this effect was greater for skMLCK-/- than wild-type muscles. After the PS, the catchlike effect was reduced in wild-type muscles but unchanged in skMLCK-/- muscles that did not display PTP. Conclusions: These data suggest that PTP interferes with the CLP during concentric force development at moderate speeds of shortening. We conclude that the physiological utility of each mechanism and their interactions provide important modulations to fast skeletal muscle function. Muscle Nerve 54: 308–316, 2016.

Original languageEnglish (US)
Pages (from-to)308-316
Number of pages9
JournalMuscle and Nerve
Volume54
Issue number2
DOIs
StatePublished - Aug 1 2016

Fingerprint

Skeletal Muscle
Muscles
Myosins
Hindlimb
Genotype
Phosphorylation

Keywords

  • catchlike property
  • concentric
  • myosin light chain kinase knockout
  • myosin phosphorylation
  • posttetanic potentiation
  • regulatory light chain

ASJC Scopus subject areas

  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)
  • Physiology

Cite this

Interaction of posttetanic potentiation and the catchlike property in mouse skeletal muscle. / Gittings, William; Bunda, Jordan; Stull, James T.; Vandenboom, Rene.

In: Muscle and Nerve, Vol. 54, No. 2, 01.08.2016, p. 308-316.

Research output: Contribution to journalArticle

Gittings, William ; Bunda, Jordan ; Stull, James T. ; Vandenboom, Rene. / Interaction of posttetanic potentiation and the catchlike property in mouse skeletal muscle. In: Muscle and Nerve. 2016 ; Vol. 54, No. 2. pp. 308-316.
@article{5e09a2f971084f179f5f4942ba623751,
title = "Interaction of posttetanic potentiation and the catchlike property in mouse skeletal muscle",
abstract = "Introduction: Posttetanic potentiation (PTP) and the catchlike property (CLP) enhance contractile function in skeletal muscle. We investigated the CLP during dynamic performance in mouse hindlimb muscles with (wild-type) and without (skMLCK-/-) the primary mechanism for PTP (myosin phosphorylation) (in vitro, 25°C). Methods: Extensor digitorum longus muscles of both genotypes were stimulated with constant frequency and catchlike trains (CFT and CLT), before and after a potentiating stimulus (PS). Results: Before the PS, the CLT increased concentric force/work relative to the CFT, but this effect was greater for skMLCK-/- than wild-type muscles. After the PS, the catchlike effect was reduced in wild-type muscles but unchanged in skMLCK-/- muscles that did not display PTP. Conclusions: These data suggest that PTP interferes with the CLP during concentric force development at moderate speeds of shortening. We conclude that the physiological utility of each mechanism and their interactions provide important modulations to fast skeletal muscle function. Muscle Nerve 54: 308–316, 2016.",
keywords = "catchlike property, concentric, myosin light chain kinase knockout, myosin phosphorylation, posttetanic potentiation, regulatory light chain",
author = "William Gittings and Jordan Bunda and Stull, {James T.} and Rene Vandenboom",
year = "2016",
month = "8",
day = "1",
doi = "10.1002/mus.25053",
language = "English (US)",
volume = "54",
pages = "308--316",
journal = "Muscle and Nerve",
issn = "0148-639X",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - Interaction of posttetanic potentiation and the catchlike property in mouse skeletal muscle

AU - Gittings, William

AU - Bunda, Jordan

AU - Stull, James T.

AU - Vandenboom, Rene

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Introduction: Posttetanic potentiation (PTP) and the catchlike property (CLP) enhance contractile function in skeletal muscle. We investigated the CLP during dynamic performance in mouse hindlimb muscles with (wild-type) and without (skMLCK-/-) the primary mechanism for PTP (myosin phosphorylation) (in vitro, 25°C). Methods: Extensor digitorum longus muscles of both genotypes were stimulated with constant frequency and catchlike trains (CFT and CLT), before and after a potentiating stimulus (PS). Results: Before the PS, the CLT increased concentric force/work relative to the CFT, but this effect was greater for skMLCK-/- than wild-type muscles. After the PS, the catchlike effect was reduced in wild-type muscles but unchanged in skMLCK-/- muscles that did not display PTP. Conclusions: These data suggest that PTP interferes with the CLP during concentric force development at moderate speeds of shortening. We conclude that the physiological utility of each mechanism and their interactions provide important modulations to fast skeletal muscle function. Muscle Nerve 54: 308–316, 2016.

AB - Introduction: Posttetanic potentiation (PTP) and the catchlike property (CLP) enhance contractile function in skeletal muscle. We investigated the CLP during dynamic performance in mouse hindlimb muscles with (wild-type) and without (skMLCK-/-) the primary mechanism for PTP (myosin phosphorylation) (in vitro, 25°C). Methods: Extensor digitorum longus muscles of both genotypes were stimulated with constant frequency and catchlike trains (CFT and CLT), before and after a potentiating stimulus (PS). Results: Before the PS, the CLT increased concentric force/work relative to the CFT, but this effect was greater for skMLCK-/- than wild-type muscles. After the PS, the catchlike effect was reduced in wild-type muscles but unchanged in skMLCK-/- muscles that did not display PTP. Conclusions: These data suggest that PTP interferes with the CLP during concentric force development at moderate speeds of shortening. We conclude that the physiological utility of each mechanism and their interactions provide important modulations to fast skeletal muscle function. Muscle Nerve 54: 308–316, 2016.

KW - catchlike property

KW - concentric

KW - myosin light chain kinase knockout

KW - myosin phosphorylation

KW - posttetanic potentiation

KW - regulatory light chain

UR - http://www.scopus.com/inward/record.url?scp=84978906936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978906936&partnerID=8YFLogxK

U2 - 10.1002/mus.25053

DO - 10.1002/mus.25053

M3 - Article

C2 - 26802366

AN - SCOPUS:84978906936

VL - 54

SP - 308

EP - 316

JO - Muscle and Nerve

JF - Muscle and Nerve

SN - 0148-639X

IS - 2

ER -