TY - GEN
T1 - Interactions of prostate cancer cells to human microvascular endothelial cells under shear stress conditions
AU - Xu, Hao
AU - Kalu, Nene
AU - Raghavan, Pavithra
AU - Kim, Myoung H.
AU - Nguyen, Kytai Truong
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2007
Y1 - 2007
N2 - The interactions between prostate cancer cells with endothelial cells in the microvessels play an important role in prostate tumor metastasis. However, not many studies have been conducted to investigate the adhesion of prostate cancer cells on vascular endothelium and related mechanisms and modulators. In this study, human microvascular endothelial cells (HMEC) grown on glass slides were exposed to two different circulating prostate cancer cells, PC-3 and PC-3ML (highly metastasis prostate cancer cells) using an in vitro parallel flow system. The gene profiles of HMEC, after exposure to prostate cancer cells, were also investigated using cDNA microarrays. It was found that PC-3ML cells had significantly higher adhesion than PC-3 cells on HMEC monolayer. In addition, PC-3ML cells trigged more gene changes of HMEC than PC-3 cells did. These results suggested that prostate cancer cells might increase their adhesion to vascular endothelial cells by affecting the endothelial cell on the gene expression level.
AB - The interactions between prostate cancer cells with endothelial cells in the microvessels play an important role in prostate tumor metastasis. However, not many studies have been conducted to investigate the adhesion of prostate cancer cells on vascular endothelium and related mechanisms and modulators. In this study, human microvascular endothelial cells (HMEC) grown on glass slides were exposed to two different circulating prostate cancer cells, PC-3 and PC-3ML (highly metastasis prostate cancer cells) using an in vitro parallel flow system. The gene profiles of HMEC, after exposure to prostate cancer cells, were also investigated using cDNA microarrays. It was found that PC-3ML cells had significantly higher adhesion than PC-3 cells on HMEC monolayer. In addition, PC-3ML cells trigged more gene changes of HMEC than PC-3 cells did. These results suggested that prostate cancer cells might increase their adhesion to vascular endothelial cells by affecting the endothelial cell on the gene expression level.
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U2 - 10.1109/EMBSW.2007.4454190
DO - 10.1109/EMBSW.2007.4454190
M3 - Conference contribution
AN - SCOPUS:48949118108
SN - 9781424416264
T3 - 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS
SP - 126
EP - 129
BT - 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS
T2 - 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS
Y2 - 11 November 2007 through 12 November 2007
ER -