Abstract
The topographic structure of Giemsa-banded (G-banded) early metaphase human chromosomes adsorbed on glass was analyzed by atomic force microscope using amplitude modulation mode (AM-AFM). Longitudinal height measurements for early metaphasic human chromosomes showed a central ridge that was further characterized by transversal height measurements. The heterochromatic regions displayed a high level of transversal symmetry, while the euchromatic ones presented several peaks across the transversal height measurements. We suggest that this central ridge and symmetry patterns point out a transitional arrangement of the early metaphase chromosome and support evidence for interchromatidal interactions prior to disjunction.
Original language | English (US) |
---|---|
Pages (from-to) | 184-189 |
Number of pages | 6 |
Journal | Journal of Molecular Recognition |
Volume | 21 |
Issue number | 3 |
DOIs | |
State | Published - May 2008 |
Externally published | Yes |
Keywords
- AFM
- Chromosome structure
- G-banding
- Interchromatidal interactions
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology