Interference of ascorbic acid in the sensitive detection of dopamine by a nonoxidative sensing approach

Shah R. Ali, Rishi R. Parajuli, Yufeng Ma, Yetunde Balogun, Huixin He

Research output: Contribution to journalArticlepeer-review

Abstract

The electrochemistry of a poly(anilineboronic acid)/carbon nanotube composite was studied in the presence of dopamine and ascorbic acid. To understand the binding affinity of dopamine and ascorbic acid to the boronic acid functional groups in the composite, the association constants between the diol groups in dopamine and ascorbic acid and the boronic acid were experimentally determined using a fluorescence-based binding assay. The results demonstrate that ascorbic acid could severely interfere with the detection of dopamine in nonoxidative boronic acid-binding approaches: Ascorbic acid was able to electrocatalytically reduce the fully oxidized polyaniline backbone during the electrochemical oxidation process; similarly to dopamine, ascorbic acid was also able to bind to the boronic acid groups through its planar diol group even though the binding affinity is much lower. The examination of the dopamine transduction mechanism and ascorbic acid interference mechanism in this nonoxidative approach will benefit the design of future boronic acid-based sensors.

Original languageEnglish (US)
Pages (from-to)12275-12281
Number of pages7
JournalJournal of Physical Chemistry B
Volume111
Issue number42
DOIs
StatePublished - Oct 25 2007
Externally publishedYes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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