Interference with function of a homeobox gene in Xenopus embryos produces malformations of the anterior spinal cord

Christopher V E Wright, Ken W Y Cho, Jane Hardwicke, Robert H. Collins, Eddy M. De Robertis

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

XIHbox 1 is expressed in a narrow band across the cervical region of Xenopus embryos. The gene produces two related proteins: "long" and "short" XIHbox 1 homeodomain proteins. Injection of antibodies to the long XIHbox 1 protein into 1-cell embryos caused a phenotype in which the anterior spinal cord was morphologically transformed into a hindbrain-like structure. This alteration was restricted to the region normally expressing long XIHbox 1 protein. Injection of long protein mRNA disrupted segmentation and tissue organization without inhibiting cell proliferation. Injection of short protein mRNA into 1-cell embryos produced spinal cord malformations similar, but not identical, to those caused by the antibodies, suggesting antagonistic roles for long and short XIHbox 1 proteins. We immunostained tadpoles carrying extended hindbrains for N-CAM and consistently found defective organization of spinal nerves over the affected region.

Original languageEnglish (US)
Pages (from-to)81-93
Number of pages13
JournalCell
Volume59
Issue number1
DOIs
StatePublished - Oct 6 1989

Fingerprint

Homeobox Genes
Xenopus
Spinal Cord
Embryonic Structures
Genes
Rhombencephalon
Proteins
Injections
Homeodomain Proteins
Spinal Nerves
Messenger RNA
Antibodies
Cell proliferation
Computer aided manufacturing
Larva
Cell Proliferation
Tissue
Phenotype

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Interference with function of a homeobox gene in Xenopus embryos produces malformations of the anterior spinal cord. / Wright, Christopher V E; Cho, Ken W Y; Hardwicke, Jane; Collins, Robert H.; De Robertis, Eddy M.

In: Cell, Vol. 59, No. 1, 06.10.1989, p. 81-93.

Research output: Contribution to journalArticle

Wright, Christopher V E ; Cho, Ken W Y ; Hardwicke, Jane ; Collins, Robert H. ; De Robertis, Eddy M. / Interference with function of a homeobox gene in Xenopus embryos produces malformations of the anterior spinal cord. In: Cell. 1989 ; Vol. 59, No. 1. pp. 81-93.
@article{8a04055f7d064ae3bf32858739d8e3f3,
title = "Interference with function of a homeobox gene in Xenopus embryos produces malformations of the anterior spinal cord",
abstract = "XIHbox 1 is expressed in a narrow band across the cervical region of Xenopus embryos. The gene produces two related proteins: {"}long{"} and {"}short{"} XIHbox 1 homeodomain proteins. Injection of antibodies to the long XIHbox 1 protein into 1-cell embryos caused a phenotype in which the anterior spinal cord was morphologically transformed into a hindbrain-like structure. This alteration was restricted to the region normally expressing long XIHbox 1 protein. Injection of long protein mRNA disrupted segmentation and tissue organization without inhibiting cell proliferation. Injection of short protein mRNA into 1-cell embryos produced spinal cord malformations similar, but not identical, to those caused by the antibodies, suggesting antagonistic roles for long and short XIHbox 1 proteins. We immunostained tadpoles carrying extended hindbrains for N-CAM and consistently found defective organization of spinal nerves over the affected region.",
author = "Wright, {Christopher V E} and Cho, {Ken W Y} and Jane Hardwicke and Collins, {Robert H.} and {De Robertis}, {Eddy M.}",
year = "1989",
month = "10",
day = "6",
doi = "10.1016/0092-8674(89)90871-4",
language = "English (US)",
volume = "59",
pages = "81--93",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - Interference with function of a homeobox gene in Xenopus embryos produces malformations of the anterior spinal cord

AU - Wright, Christopher V E

AU - Cho, Ken W Y

AU - Hardwicke, Jane

AU - Collins, Robert H.

AU - De Robertis, Eddy M.

PY - 1989/10/6

Y1 - 1989/10/6

N2 - XIHbox 1 is expressed in a narrow band across the cervical region of Xenopus embryos. The gene produces two related proteins: "long" and "short" XIHbox 1 homeodomain proteins. Injection of antibodies to the long XIHbox 1 protein into 1-cell embryos caused a phenotype in which the anterior spinal cord was morphologically transformed into a hindbrain-like structure. This alteration was restricted to the region normally expressing long XIHbox 1 protein. Injection of long protein mRNA disrupted segmentation and tissue organization without inhibiting cell proliferation. Injection of short protein mRNA into 1-cell embryos produced spinal cord malformations similar, but not identical, to those caused by the antibodies, suggesting antagonistic roles for long and short XIHbox 1 proteins. We immunostained tadpoles carrying extended hindbrains for N-CAM and consistently found defective organization of spinal nerves over the affected region.

AB - XIHbox 1 is expressed in a narrow band across the cervical region of Xenopus embryos. The gene produces two related proteins: "long" and "short" XIHbox 1 homeodomain proteins. Injection of antibodies to the long XIHbox 1 protein into 1-cell embryos caused a phenotype in which the anterior spinal cord was morphologically transformed into a hindbrain-like structure. This alteration was restricted to the region normally expressing long XIHbox 1 protein. Injection of long protein mRNA disrupted segmentation and tissue organization without inhibiting cell proliferation. Injection of short protein mRNA into 1-cell embryos produced spinal cord malformations similar, but not identical, to those caused by the antibodies, suggesting antagonistic roles for long and short XIHbox 1 proteins. We immunostained tadpoles carrying extended hindbrains for N-CAM and consistently found defective organization of spinal nerves over the affected region.

UR - http://www.scopus.com/inward/record.url?scp=0024436103&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024436103&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(89)90871-4

DO - 10.1016/0092-8674(89)90871-4

M3 - Article

C2 - 2477158

AN - SCOPUS:0024436103

VL - 59

SP - 81

EP - 93

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

ER -