Interleukin-1β converting enzyme in murine Langerhans cells and epidermal-derived dendritic cell lines

Kiyoshi Ariizumi, Toshiyuki Kitajima, Paul R. Bergstresser, Akira Takashima

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Interleukin (IL)-1β plays an essential role in the induction of T cell-mediated immune responses in skin. Langerhans cells (LC), which constitutively express IL-1β mRNA, have been assumed to be the primary source of IL-1β in murine epidermis. The purpose of this study was to determine whether LC express mRNA for the IL-1β converting enzyme (ICE), a protease that is required for processing pro-IL-1β into an active form. Here, we report that both IL-1β and ICE mRNA are expressed by the Ia+ population (i.e. LC) in murine epidermis. Moreover, murine epidermal-derived DC lines (XS series) also express both IL-1β and ICE mRNA, and they secrete relatively large amounts of IL-1β following lipopolysaccharide (LPS) stimulation. Finally, LPS-triggered IL-1β secretion by XS cells is blocked almost completely by the ICE inhibitor acetyl-Tyr-Val-Ala-Asp-CH2OC(O)-[2,6-(CF3)2]Ph. These results demonstrate that LC are the primary source of IL-1β within the epidermis, and suggest that the proinflammatory role of IL-1β may be regulated pharmacologically by ICE inhibitors in vivo.

Original languageEnglish (US)
Pages (from-to)2137-2141
Number of pages5
JournalEuropean Journal of Immunology
Volume25
Issue number8
DOIs
StatePublished - Aug 1995

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Caspase 1
Langerhans Cells
Interleukin-1
Cell Line
Epidermis
Messenger RNA
Enzyme Inhibitors
Lipopolysaccharides
Enzymes

Keywords

  • Interleukin-1β
  • Interleukin-1β converting enzyme
  • Langerhans cells

ASJC Scopus subject areas

  • Immunology

Cite this

Interleukin-1β converting enzyme in murine Langerhans cells and epidermal-derived dendritic cell lines. / Ariizumi, Kiyoshi; Kitajima, Toshiyuki; Bergstresser, Paul R.; Takashima, Akira.

In: European Journal of Immunology, Vol. 25, No. 8, 08.1995, p. 2137-2141.

Research output: Contribution to journalArticle

Ariizumi, Kiyoshi ; Kitajima, Toshiyuki ; Bergstresser, Paul R. ; Takashima, Akira. / Interleukin-1β converting enzyme in murine Langerhans cells and epidermal-derived dendritic cell lines. In: European Journal of Immunology. 1995 ; Vol. 25, No. 8. pp. 2137-2141.
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