TY - JOUR
T1 - Interleukin-1β induces expression of neuropeptide Y in primary astrocyte cultures in a cytokine-specific manner
T2 - Induction in human but not rat astrocytes
AU - Barnea, Ayalla
AU - Roberts, Jodie
AU - Keller, Patrick
AU - Word, R. Ann
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2001/3/30
Y1 - 2001/3/30
N2 - Previous studies have demonstrated that astrocyte cultures express neuropeptide Y (NPY) in a regulated manner, namely, phorbol ester leads to an increase in proNPY-mRNA and NPY production. In this respect, the behavior of astrocytes derived from the human fetal or rat neonatal brain is similar (Regul. Pept. 75 (1998) 293). Since astrocytes can be exposed to high levels of IL-1β, we addressed the question: Does IL-1β regulate NPY expression by the astrocytes? Primary astrocytes derived from the human fetal or rat neonatal cortex were cultured in serum-free medium. IL-1β, but not IL-6 or TNF-α, led to an increase in NPY production dose-dependently. IL-1β action manifested in the human but not in the rat astrocytes and it was completely abolished by IL-1 receptor antagonist. The responsiveness to IL-1β did not diminish upon sub-culture of the astrocytes (five passages). In addition, IL-1β led to an increase in the abundance of proNPY-mRNA, which was preceded by a rapid and transient increase in cFos-mRNA and a rapid and sustained increase in JunB-mRNA. In contrast to cFos/JunB, IL-1β did not alter the abundance of cJun-mRNA. In summary, we demonstrate that IL-1β induction of NPY expression in astrocytes is species- and cytokine-specific and that IL-1 receptor is involved. Moreover, induction of NPY expression is preceded by a rapid increase in the expression of two transcription factors (cFos, JunB) that have been previously (Oncogene 9 (1994) 2369; J. Neurochem. 70 (1998) 1887) implicated in transcriptional regulation of the human NPY gene.
AB - Previous studies have demonstrated that astrocyte cultures express neuropeptide Y (NPY) in a regulated manner, namely, phorbol ester leads to an increase in proNPY-mRNA and NPY production. In this respect, the behavior of astrocytes derived from the human fetal or rat neonatal brain is similar (Regul. Pept. 75 (1998) 293). Since astrocytes can be exposed to high levels of IL-1β, we addressed the question: Does IL-1β regulate NPY expression by the astrocytes? Primary astrocytes derived from the human fetal or rat neonatal cortex were cultured in serum-free medium. IL-1β, but not IL-6 or TNF-α, led to an increase in NPY production dose-dependently. IL-1β action manifested in the human but not in the rat astrocytes and it was completely abolished by IL-1 receptor antagonist. The responsiveness to IL-1β did not diminish upon sub-culture of the astrocytes (five passages). In addition, IL-1β led to an increase in the abundance of proNPY-mRNA, which was preceded by a rapid and transient increase in cFos-mRNA and a rapid and sustained increase in JunB-mRNA. In contrast to cFos/JunB, IL-1β did not alter the abundance of cJun-mRNA. In summary, we demonstrate that IL-1β induction of NPY expression in astrocytes is species- and cytokine-specific and that IL-1 receptor is involved. Moreover, induction of NPY expression is preceded by a rapid increase in the expression of two transcription factors (cFos, JunB) that have been previously (Oncogene 9 (1994) 2369; J. Neurochem. 70 (1998) 1887) implicated in transcriptional regulation of the human NPY gene.
KW - AP-1 complex
KW - Fos
KW - Jun
KW - ProNPY-mRNA
KW - Transcriptional regulation
KW - Tumor necrosis factor
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U2 - 10.1016/S0006-8993(01)02141-2
DO - 10.1016/S0006-8993(01)02141-2
M3 - Article
C2 - 11277982
AN - SCOPUS:0035970282
SN - 0006-8993
VL - 896
SP - 137
EP - 145
JO - Brain Research
JF - Brain Research
IS - 1-2
ER -