Interleukin 1 increases the production of endothelin-1 by cultured endothelial cells

Masao Yoshizumi, Hiroki Kurihara, Toshisuke Morita, Takahiro Yamashita, Yoshio Oh-hashi, Takao Sugiyama, Fumimaro Takaku, Masashi Yanagisawa, Tomoh Masaki, Yoshio Yazaki

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Abstract

We examined the effect of human recombinant interleukin 1 (IL-1) on the production of endothelin-1 by cultured porcine endothelial cells. The induction of endothelin-1 mRNA began within 1 hr of exposure to IL-1, showed twin peaks at 4 and 24 hr, and declined thereafter. Enzyme-linked immunosorbent assay revealed that the amount of endothelin-1 peptide in conditioned media was also increased by IL-1 in a dose- and time-dependent manner. Our results suggested that IL-1, a macrophage-derived cytokine, may affect the contraction and proliferation of vascular smooth muscle cells by stimulating the production of endothelin by endothelial cells.

Original languageEnglish (US)
Pages (from-to)324-329
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume166
Issue number1
DOIs
Publication statusPublished - Jan 15 1990

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Yoshizumi, M., Kurihara, H., Morita, T., Yamashita, T., Oh-hashi, Y., Sugiyama, T., ... Yazaki, Y. (1990). Interleukin 1 increases the production of endothelin-1 by cultured endothelial cells. Biochemical and Biophysical Research Communications, 166(1), 324-329. https://doi.org/10.1016/0006-291X(90)91948-R