Interleukin 1 increases the production of endothelin-1 by cultured endothelial cells

Masao Yoshizumi; Hiroki Kurihara; Toshisuke Morita; Takahiro Yamashita; Yoshio Oh-hashi; Takao Sugiyama; Fumimaro Takaku; Masashi Yanagisawa; Tomoh Masaki; Yoshio Yazaki

doi:10.1016/0006-291X(90)91948-R

Interleukin 1 increases the production of endothelin-1 by cultured endothelial cells

Masao Yoshizumi, Hiroki Kurihara, Toshisuke Morita, Takahiro Yamashita, Yoshio Oh-hashi, Takao Sugiyama, Fumimaro Takaku, Masashi Yanagisawa, Tomoh Masaki, Yoshio Yazaki

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Abstract

We examined the effect of human recombinant interleukin 1 (IL-1) on the production of endothelin-1 by cultured porcine endothelial cells. The induction of endothelin-1 mRNA began within 1 hr of exposure to IL-1, showed twin peaks at 4 and 24 hr, and declined thereafter. Enzyme-linked immunosorbent assay revealed that the amount of endothelin-1 peptide in conditioned media was also increased by IL-1 in a dose- and time-dependent manner. Our results suggested that IL-1, a macrophage-derived cytokine, may affect the contraction and proliferation of vascular smooth muscle cells by stimulating the production of endothelin by endothelial cells.

Original language	English (US)
Pages (from-to)	324-329
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	166
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(90)91948-R
State	Published - Jan 15 1990

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(90)91948-R

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@article{06e1460dea50466ba2600be4370f6eba,

title = "Interleukin 1 increases the production of endothelin-1 by cultured endothelial cells",

abstract = "We examined the effect of human recombinant interleukin 1 (IL-1) on the production of endothelin-1 by cultured porcine endothelial cells. The induction of endothelin-1 mRNA began within 1 hr of exposure to IL-1, showed twin peaks at 4 and 24 hr, and declined thereafter. Enzyme-linked immunosorbent assay revealed that the amount of endothelin-1 peptide in conditioned media was also increased by IL-1 in a dose- and time-dependent manner. Our results suggested that IL-1, a macrophage-derived cytokine, may affect the contraction and proliferation of vascular smooth muscle cells by stimulating the production of endothelin by endothelial cells.",

author = "Masao Yoshizumi and Hiroki Kurihara and Toshisuke Morita and Takahiro Yamashita and Yoshio Oh-hashi and Takao Sugiyama and Fumimaro Takaku and Masashi Yanagisawa and Tomoh Masaki and Yoshio Yazaki",

note = "Funding Information: Am: This work was supported by a grant-in-aid for scientific research fran the Ministry of Education, Science and Culture and by a grant from the Research Program on Cell Calcium Signals in the Cardiovascular System, Japan.",

year = "1990",

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doi = "10.1016/0006-291X(90)91948-R",

language = "English (US)",

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T1 - Interleukin 1 increases the production of endothelin-1 by cultured endothelial cells

AU - Yoshizumi, Masao

AU - Kurihara, Hiroki

AU - Morita, Toshisuke

AU - Yamashita, Takahiro

AU - Oh-hashi, Yoshio

AU - Sugiyama, Takao

AU - Takaku, Fumimaro

AU - Yanagisawa, Masashi

AU - Masaki, Tomoh

AU - Yazaki, Yoshio

N1 - Funding Information: Am: This work was supported by a grant-in-aid for scientific research fran the Ministry of Education, Science and Culture and by a grant from the Research Program on Cell Calcium Signals in the Cardiovascular System, Japan.

PY - 1990/1/15

Y1 - 1990/1/15

N2 - We examined the effect of human recombinant interleukin 1 (IL-1) on the production of endothelin-1 by cultured porcine endothelial cells. The induction of endothelin-1 mRNA began within 1 hr of exposure to IL-1, showed twin peaks at 4 and 24 hr, and declined thereafter. Enzyme-linked immunosorbent assay revealed that the amount of endothelin-1 peptide in conditioned media was also increased by IL-1 in a dose- and time-dependent manner. Our results suggested that IL-1, a macrophage-derived cytokine, may affect the contraction and proliferation of vascular smooth muscle cells by stimulating the production of endothelin by endothelial cells.

AB - We examined the effect of human recombinant interleukin 1 (IL-1) on the production of endothelin-1 by cultured porcine endothelial cells. The induction of endothelin-1 mRNA began within 1 hr of exposure to IL-1, showed twin peaks at 4 and 24 hr, and declined thereafter. Enzyme-linked immunosorbent assay revealed that the amount of endothelin-1 peptide in conditioned media was also increased by IL-1 in a dose- and time-dependent manner. Our results suggested that IL-1, a macrophage-derived cytokine, may affect the contraction and proliferation of vascular smooth muscle cells by stimulating the production of endothelin by endothelial cells.

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Interleukin 1 increases the production of endothelin-1 by cultured endothelial cells

Abstract

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