Interleukin-10 Receptor Signaling through STAT-3 Regulates the Apoptosis of Retinal Ganglion Cells in Response to Stress

Zachary S. Boyd, Aleksie Kriatchko, Junjie Yang, Neeraj Agarwal, Martin B. Wax, Rajkumar V. Patil

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

PURPOSE. Interleukin (IL)-10 has recently been shown to promote survival of neurons and glia. The purpose of this report is to investigate whether IL-10 has any role in protecting retinal ganglion cells (RGCs) from death under conditions in which growth factors are removed, or in which oxidative stress is present. Signal transduction pathways that activate IL-10 signaling in RGCs were studied in both stress conditions. METHODS. Effects of various interleukins on the viability of the RGC cell line was determined, and apoptotic cells were quantified. Immunoblot analysis was preformed to identify the IL-10 receptor (IL-10R) and phosphorylated or nonphosphorylated Akt and STAT-3 proteins in RGC extracts. Immunohistochemistry was performed on the rat retinal sections to identify native IL-10R. RESULTS. Apoptosis of RGCs in the absence of growth factors with or without dexamethasone (1 μM) occurred in 68.5% ± 3.4% and 53.4% ± 2.6% of cells, respectively, after 96 hours. Addition of IL-10 at a concentration of 50 ng/mL significantly reduced the apoptotic population of RGCs to 28.2% ± 2.3% in the absence of growth factors with dexamethasone and to 31% ± 2.7% in the absence of growth factors alone. RGCs as well as native retina expressed functional IL-10R as determined by immunoblot analysis and by the ability of IL-10 to phosphorylate Stat-3. However, IL-10 failed to phosphorylate Akt in these cells. CONCLUSIONS. IL-10 caused a 59% and 42% reduction in the apoptotic population of serum-deprived cells with and without dexamethasone treatment, respectively. These observations establish that activation of IL-10R promotes survival of RGCs and this survival-promoting activity is due to IL-10 signaling through the Stat-3 pathway, which inhibits the cell death and not through the Akt cell survival pathway.

Original languageEnglish (US)
Pages (from-to)5206-5211
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume44
Issue number12
DOIs
StatePublished - Dec 2003

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Interleukin-10 Receptors
Retinal Ganglion Cells
Interleukin-10
Apoptosis
Interleukins
Intercellular Signaling Peptides and Proteins
Dexamethasone
Cell Survival
Cell Death
Cell Extracts
Neuroglia
Population
Retina
Signal Transduction
Oxidative Stress
Immunohistochemistry
Neurons
Cell Line

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Interleukin-10 Receptor Signaling through STAT-3 Regulates the Apoptosis of Retinal Ganglion Cells in Response to Stress. / Boyd, Zachary S.; Kriatchko, Aleksie; Yang, Junjie; Agarwal, Neeraj; Wax, Martin B.; Patil, Rajkumar V.

In: Investigative Ophthalmology and Visual Science, Vol. 44, No. 12, 12.2003, p. 5206-5211.

Research output: Contribution to journalArticle

Boyd, Zachary S. ; Kriatchko, Aleksie ; Yang, Junjie ; Agarwal, Neeraj ; Wax, Martin B. ; Patil, Rajkumar V. / Interleukin-10 Receptor Signaling through STAT-3 Regulates the Apoptosis of Retinal Ganglion Cells in Response to Stress. In: Investigative Ophthalmology and Visual Science. 2003 ; Vol. 44, No. 12. pp. 5206-5211.
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abstract = "PURPOSE. Interleukin (IL)-10 has recently been shown to promote survival of neurons and glia. The purpose of this report is to investigate whether IL-10 has any role in protecting retinal ganglion cells (RGCs) from death under conditions in which growth factors are removed, or in which oxidative stress is present. Signal transduction pathways that activate IL-10 signaling in RGCs were studied in both stress conditions. METHODS. Effects of various interleukins on the viability of the RGC cell line was determined, and apoptotic cells were quantified. Immunoblot analysis was preformed to identify the IL-10 receptor (IL-10R) and phosphorylated or nonphosphorylated Akt and STAT-3 proteins in RGC extracts. Immunohistochemistry was performed on the rat retinal sections to identify native IL-10R. RESULTS. Apoptosis of RGCs in the absence of growth factors with or without dexamethasone (1 μM) occurred in 68.5{\%} ± 3.4{\%} and 53.4{\%} ± 2.6{\%} of cells, respectively, after 96 hours. Addition of IL-10 at a concentration of 50 ng/mL significantly reduced the apoptotic population of RGCs to 28.2{\%} ± 2.3{\%} in the absence of growth factors with dexamethasone and to 31{\%} ± 2.7{\%} in the absence of growth factors alone. RGCs as well as native retina expressed functional IL-10R as determined by immunoblot analysis and by the ability of IL-10 to phosphorylate Stat-3. However, IL-10 failed to phosphorylate Akt in these cells. CONCLUSIONS. IL-10 caused a 59{\%} and 42{\%} reduction in the apoptotic population of serum-deprived cells with and without dexamethasone treatment, respectively. These observations establish that activation of IL-10R promotes survival of RGCs and this survival-promoting activity is due to IL-10 signaling through the Stat-3 pathway, which inhibits the cell death and not through the Akt cell survival pathway.",
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T1 - Interleukin-10 Receptor Signaling through STAT-3 Regulates the Apoptosis of Retinal Ganglion Cells in Response to Stress

AU - Boyd, Zachary S.

AU - Kriatchko, Aleksie

AU - Yang, Junjie

AU - Agarwal, Neeraj

AU - Wax, Martin B.

AU - Patil, Rajkumar V.

PY - 2003/12

Y1 - 2003/12

N2 - PURPOSE. Interleukin (IL)-10 has recently been shown to promote survival of neurons and glia. The purpose of this report is to investigate whether IL-10 has any role in protecting retinal ganglion cells (RGCs) from death under conditions in which growth factors are removed, or in which oxidative stress is present. Signal transduction pathways that activate IL-10 signaling in RGCs were studied in both stress conditions. METHODS. Effects of various interleukins on the viability of the RGC cell line was determined, and apoptotic cells were quantified. Immunoblot analysis was preformed to identify the IL-10 receptor (IL-10R) and phosphorylated or nonphosphorylated Akt and STAT-3 proteins in RGC extracts. Immunohistochemistry was performed on the rat retinal sections to identify native IL-10R. RESULTS. Apoptosis of RGCs in the absence of growth factors with or without dexamethasone (1 μM) occurred in 68.5% ± 3.4% and 53.4% ± 2.6% of cells, respectively, after 96 hours. Addition of IL-10 at a concentration of 50 ng/mL significantly reduced the apoptotic population of RGCs to 28.2% ± 2.3% in the absence of growth factors with dexamethasone and to 31% ± 2.7% in the absence of growth factors alone. RGCs as well as native retina expressed functional IL-10R as determined by immunoblot analysis and by the ability of IL-10 to phosphorylate Stat-3. However, IL-10 failed to phosphorylate Akt in these cells. CONCLUSIONS. IL-10 caused a 59% and 42% reduction in the apoptotic population of serum-deprived cells with and without dexamethasone treatment, respectively. These observations establish that activation of IL-10R promotes survival of RGCs and this survival-promoting activity is due to IL-10 signaling through the Stat-3 pathway, which inhibits the cell death and not through the Akt cell survival pathway.

AB - PURPOSE. Interleukin (IL)-10 has recently been shown to promote survival of neurons and glia. The purpose of this report is to investigate whether IL-10 has any role in protecting retinal ganglion cells (RGCs) from death under conditions in which growth factors are removed, or in which oxidative stress is present. Signal transduction pathways that activate IL-10 signaling in RGCs were studied in both stress conditions. METHODS. Effects of various interleukins on the viability of the RGC cell line was determined, and apoptotic cells were quantified. Immunoblot analysis was preformed to identify the IL-10 receptor (IL-10R) and phosphorylated or nonphosphorylated Akt and STAT-3 proteins in RGC extracts. Immunohistochemistry was performed on the rat retinal sections to identify native IL-10R. RESULTS. Apoptosis of RGCs in the absence of growth factors with or without dexamethasone (1 μM) occurred in 68.5% ± 3.4% and 53.4% ± 2.6% of cells, respectively, after 96 hours. Addition of IL-10 at a concentration of 50 ng/mL significantly reduced the apoptotic population of RGCs to 28.2% ± 2.3% in the absence of growth factors with dexamethasone and to 31% ± 2.7% in the absence of growth factors alone. RGCs as well as native retina expressed functional IL-10R as determined by immunoblot analysis and by the ability of IL-10 to phosphorylate Stat-3. However, IL-10 failed to phosphorylate Akt in these cells. CONCLUSIONS. IL-10 caused a 59% and 42% reduction in the apoptotic population of serum-deprived cells with and without dexamethasone treatment, respectively. These observations establish that activation of IL-10R promotes survival of RGCs and this survival-promoting activity is due to IL-10 signaling through the Stat-3 pathway, which inhibits the cell death and not through the Akt cell survival pathway.

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