Interleukin-15 expression by human endothelial cells

Up-regulation by ultraviolet B and psoralen plus ultraviolet A treatment

M. Mohamadzadeh, M. J. McGuire, I. Dougherty, Ponciano D Cruz

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The purposes of the present study were to determine whether endothelial cells express IL-15 and to evaluate effects of ultraviolet B (UVB) and 8-methoxypsoralens plus UVA (PUVA) on such expression. Cultured human endothelial cells derived from dermis or umbilical veins were subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblot analyses for the detection of IL-15 mRNA and protein, respectively. Both dermal and umbilical vein endothelial cells were shown to express IL-15 mRNA and protein, and these markers were upregulated following UVB or PUVA treatment (but not by UVA or 8-methoxypsoralens alone). Also using RT-PCR, dermal and umbilical vein endothelial cells were shown to express IL-2Rγc mRNA. These results expand the sources of IL-15 in skin to include keratinocytes, dermal fibroblasts, and now endothelial cells. That IL-15 from all three skin cells can be upregulated by UV treatment suggests a role for this cytokine in photosensitive disorders. Finally, the possibility of an autocrine effect of IL-15 on endothelial cells is raised by the expression of IL-2Rγc in these cells.

Original languageEnglish (US)
Pages (from-to)17-21
Number of pages5
JournalPhotodermatology Photoimmunology and Photomedicine
Volume12
Issue number1
StatePublished - 1996

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Ficusin
Interleukin-15
Up-Regulation
Endothelial Cells
Umbilical Veins
Skin
Methoxsalen
Reverse Transcriptase Polymerase Chain Reaction
Messenger RNA
Dermis
Keratinocytes
human IL15 protein
Proteins
Fibroblasts
Cytokines

Keywords

  • Endothelial cell
  • IL-15
  • IL-2Rγc
  • PUVA
  • UVB radiation

ASJC Scopus subject areas

  • Dermatology
  • Immunology

Cite this

Interleukin-15 expression by human endothelial cells : Up-regulation by ultraviolet B and psoralen plus ultraviolet A treatment. / Mohamadzadeh, M.; McGuire, M. J.; Dougherty, I.; Cruz, Ponciano D.

In: Photodermatology Photoimmunology and Photomedicine, Vol. 12, No. 1, 1996, p. 17-21.

Research output: Contribution to journalArticle

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AB - The purposes of the present study were to determine whether endothelial cells express IL-15 and to evaluate effects of ultraviolet B (UVB) and 8-methoxypsoralens plus UVA (PUVA) on such expression. Cultured human endothelial cells derived from dermis or umbilical veins were subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblot analyses for the detection of IL-15 mRNA and protein, respectively. Both dermal and umbilical vein endothelial cells were shown to express IL-15 mRNA and protein, and these markers were upregulated following UVB or PUVA treatment (but not by UVA or 8-methoxypsoralens alone). Also using RT-PCR, dermal and umbilical vein endothelial cells were shown to express IL-2Rγc mRNA. These results expand the sources of IL-15 in skin to include keratinocytes, dermal fibroblasts, and now endothelial cells. That IL-15 from all three skin cells can be upregulated by UV treatment suggests a role for this cytokine in photosensitive disorders. Finally, the possibility of an autocrine effect of IL-15 on endothelial cells is raised by the expression of IL-2Rγc in these cells.

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