Interleukin 2 modulates intestinal epithelial cell function in vitro 1

Axel U. Dignass, Daniel K. Podolsky

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Although interleukin 2 (IL-2) has been presumed to have a highly circumscribed, range of target cells limited largely to classic immune cell populations, the presence of functional IL-2 receptors in rat epithelial cell lines has recently been demonstrated. Limited information is available about the functional effects of IL-2 on intestinal epithelial cells. The effect of recombinant IL-2 on intestinal epithelial cell migration was assessed using a previously described in vitro model of epithelial restitution by quantitation of cells migrating into standard wounds established in confluent IEC-6 cell monolayers. Transforming growth factor β content was assessed by Northern blot and bioassay. Exogenous IL-2 enhanced epithelial cell restitution in vitro on average 3.8-fold; this effect was independent of cell proliferation. Enhancement of restitution through IL-2 could be completely blocked through antibodies directed against TGFβ1 and interleukin-2 receptor, indicating that stimulation of epithelial cell restitution is specifically enhanced by interleukin-2 and mediated through a TGFβ-dependent pathway. In addition, increased expression of TGFβ1 mRNA and increased levels of bioactive TGFβ peptide in wounded monolayers treated with IL-2 compared to unwounded monolayers cultured in serum-deprived medium alone support the notion that enhancement of epithelial cell restitution in vitro is mediated through a TGFβ-dependent pathway. These studies suggest that IL-2, a potent cytokine whose biological origin and targets have been presumed to be largely limited to lymphocyte and macrophage populations, may play a role in preserving the integrity of the intestinal epithelium following various forms of injuries.

Original languageEnglish (US)
Pages (from-to)422-429
Number of pages8
JournalExperimental Cell Research
Volume225
Issue number2
DOIs
StatePublished - Jun 15 1996

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Interleukin-2
Epithelial Cells
Interleukin-2 Receptors
In Vitro Techniques
Wounds and Injuries
Transforming Growth Factors
Intestinal Mucosa
Biological Assay
Northern Blotting
Population
Cell Movement
Macrophages
Cell Proliferation
Lymphocytes
Cytokines
Cell Line
Messenger RNA
Peptides
Antibodies
Serum

ASJC Scopus subject areas

  • Cell Biology

Cite this

Interleukin 2 modulates intestinal epithelial cell function in vitro 1. / Dignass, Axel U.; Podolsky, Daniel K.

In: Experimental Cell Research, Vol. 225, No. 2, 15.06.1996, p. 422-429.

Research output: Contribution to journalArticle

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