Interleukin-2 Receptor β Subunit-dependent and -independent Regulation of Intestinal Epithelial Tight Junctions

Raisuke Nishiyama, Takanori Sakaguchi, Tetsushi Kinugasa, Xiubin Gu, Richard P. MacDermott, Daniel K. Podolsky, Hans Christian Reinecker

Research output: Contribution to journalArticle

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Abstract

Interleukin (IL)-15 is able to regulate tight junction formation in intestinal epithelial cells. However, the mechanisms that regulate the intestinal barrier function in response to IL-15 and the involved subunits of the IL-15 ligand-receptor system are unknown. We determined the IL-2Rβ subunit and IL-15-dependent regulation of tight junction-associated proteins in the human intestinal epithelial cell line T-84. The IL-2Rβ subunit was expressed and induced signal transduction in caveolin enriched rafts in intestinal epithelial cells. IL-15-mediated tightening of intestinal epithelial monolayers correlated with the enhanced recruitment of tight junction proteins into Triton X-100-insoluble protein fractions. IL-15-mediated up-regulation of ZO-1 and ZO-2 expression was independent of the IL-2Rβ subunit, whereas the phosphorylation of occludin and enhanced membrane association of claudin-1 and claudin-2 by IL-15 required the presence of the IL-2Rβ subunit. Recruitment of claudins and hyperphosphorylated occludin into tight junctions resulted in a more marked induction of tight junction formation in intestinal epithelial cells than the up-regulation of ZO-1 and ZO-2 by itself. The regulation of the intestinal epithelial barrier function by IL-15 involves IL-2Rβ-dependent and -independent signaling pathways leading to the recruitment of claudins, hyperphosphorylated occludin, ZO-1, and ZO-2 into the tight junctional protein complex.

Original languageEnglish (US)
Pages (from-to)35571-35580
Number of pages10
JournalJournal of Biological Chemistry
Volume276
Issue number38
DOIs
StatePublished - Sep 21 2001

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Interleukin-15
Tight Junctions
Interleukin-2 Receptors
Interleukins
Occludin
Epithelial Cells
Claudins
Tight Junction Proteins
Interleukin-15 Receptors
Claudin-2
Up-Regulation
Claudin-1
Caveolins
Octoxynol
Signal transduction
Phosphorylation
Proteins
Signal Transduction
Monolayers
Ligands

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interleukin-2 Receptor β Subunit-dependent and -independent Regulation of Intestinal Epithelial Tight Junctions. / Nishiyama, Raisuke; Sakaguchi, Takanori; Kinugasa, Tetsushi; Gu, Xiubin; MacDermott, Richard P.; Podolsky, Daniel K.; Reinecker, Hans Christian.

In: Journal of Biological Chemistry, Vol. 276, No. 38, 21.09.2001, p. 35571-35580.

Research output: Contribution to journalArticle

Nishiyama, Raisuke ; Sakaguchi, Takanori ; Kinugasa, Tetsushi ; Gu, Xiubin ; MacDermott, Richard P. ; Podolsky, Daniel K. ; Reinecker, Hans Christian. / Interleukin-2 Receptor β Subunit-dependent and -independent Regulation of Intestinal Epithelial Tight Junctions. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 38. pp. 35571-35580.
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