Interleukin (IL)-15 promotes the growth of murine epidermal γδ T cells by a mechanism involving the β- and γ(c)-chains of the IL-2 receptor

D. Edelbaum, M. Mohamadzadeh, P. R. Bergstresser, K. Sugamura, A. Takashima

Research output: Contribution to journalArticle

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Abstract

Dendritic epidermal T cells (DETC) are skin-specific members of the epithelial γδ T-cell family in mice. We have reported previously that the growth of DETC is promoted by interleukin (IL)-2 in an autocrine fashion, or by IL-7, which is secreted by neighboring keratinocytes. Here we report that DETC growth is promoted by IL-15, a newly discovered T-cell growth factor that is produced in lymphoid as well as nonlymphoid tissues. Recombinant IL-15 promoted the growth of the 7-17 DETC line in a time- and dose-dependent fashion. Using monoclonal antibodies against α-, β-, or γ(c)-chains of the IL-2 receptor complex, we observed that the combination of anti-β chain and anti-γ(c) chain antibodies blocked IL-15 responsiveness completely, whereas anti-α chain had no effect. These results indicate that this γδ T-cell line uses the β/γ(c) heterodimer for proliferative responses to IL-15. Antibodies against IL-2 or IL-7 did not block IL-15-driven proliferation of 7-17 DETC, indicating that IL-15 promotes their growth in an IL-2- and IL-7-independent manner. Both the surface expression of β/γ(c) heterodimers and the IL-15 responsiveness of 7-17 DETC were highest 1 to 8 days after concanavalin A stimulation, and both declined substantially 21 days after stimulation, illustrating regulation by the state of cell activation. Working with epidermal cells that were freshly procured from CBA mice, we noted that IL-15 promoted concanavalin-A-triggered growth of Thy-1+ cells (i.e., DETC), but not of the Thy-1- cells. The γ(c)-chain was not expressed by freshly procured DETC, becoming detectable within 48 h after concanavalin A stimulation. We propose that IL-15 facilitates the growth of epithelial γδ T cells by a β/γ(c) receptor-dependent mechanism.

Original languageEnglish (US)
Pages (from-to)837-843
Number of pages7
JournalJournal of Investigative Dermatology
Volume105
Issue number6
StatePublished - 1995

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Interleukin-15
T-cells
Interleukin-2 Receptors
T-Lymphocytes
Growth
Interleukin-7
Interleukin-2
Concanavalin A
Epithelial Cells
Cell Line
Inbred CBA Mouse
Langerhans Cells
Antibodies
Cell growth
Keratinocytes
Skin
Chemical activation
Monoclonal Antibodies

ASJC Scopus subject areas

  • Dermatology

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Interleukin (IL)-15 promotes the growth of murine epidermal γδ T cells by a mechanism involving the β- and γ(c)-chains of the IL-2 receptor. / Edelbaum, D.; Mohamadzadeh, M.; Bergstresser, P. R.; Sugamura, K.; Takashima, A.

In: Journal of Investigative Dermatology, Vol. 105, No. 6, 1995, p. 837-843.

Research output: Contribution to journalArticle

Edelbaum, D. ; Mohamadzadeh, M. ; Bergstresser, P. R. ; Sugamura, K. ; Takashima, A. / Interleukin (IL)-15 promotes the growth of murine epidermal γδ T cells by a mechanism involving the β- and γ(c)-chains of the IL-2 receptor. In: Journal of Investigative Dermatology. 1995 ; Vol. 105, No. 6. pp. 837-843.
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T1 - Interleukin (IL)-15 promotes the growth of murine epidermal γδ T cells by a mechanism involving the β- and γ(c)-chains of the IL-2 receptor

AU - Edelbaum, D.

AU - Mohamadzadeh, M.

AU - Bergstresser, P. R.

AU - Sugamura, K.

AU - Takashima, A.

PY - 1995

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AB - Dendritic epidermal T cells (DETC) are skin-specific members of the epithelial γδ T-cell family in mice. We have reported previously that the growth of DETC is promoted by interleukin (IL)-2 in an autocrine fashion, or by IL-7, which is secreted by neighboring keratinocytes. Here we report that DETC growth is promoted by IL-15, a newly discovered T-cell growth factor that is produced in lymphoid as well as nonlymphoid tissues. Recombinant IL-15 promoted the growth of the 7-17 DETC line in a time- and dose-dependent fashion. Using monoclonal antibodies against α-, β-, or γ(c)-chains of the IL-2 receptor complex, we observed that the combination of anti-β chain and anti-γ(c) chain antibodies blocked IL-15 responsiveness completely, whereas anti-α chain had no effect. These results indicate that this γδ T-cell line uses the β/γ(c) heterodimer for proliferative responses to IL-15. Antibodies against IL-2 or IL-7 did not block IL-15-driven proliferation of 7-17 DETC, indicating that IL-15 promotes their growth in an IL-2- and IL-7-independent manner. Both the surface expression of β/γ(c) heterodimers and the IL-15 responsiveness of 7-17 DETC were highest 1 to 8 days after concanavalin A stimulation, and both declined substantially 21 days after stimulation, illustrating regulation by the state of cell activation. Working with epidermal cells that were freshly procured from CBA mice, we noted that IL-15 promoted concanavalin-A-triggered growth of Thy-1+ cells (i.e., DETC), but not of the Thy-1- cells. The γ(c)-chain was not expressed by freshly procured DETC, becoming detectable within 48 h after concanavalin A stimulation. We propose that IL-15 facilitates the growth of epithelial γδ T cells by a β/γ(c) receptor-dependent mechanism.

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