Intermediate filament and cross-linked envelope expression in human lung tumor cell lines

S. P. Banks-Schlegel, A. F. Gazdar, C. C. Harris

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Human lung tumor cell lines established from the major histological types of lung cancer were examined by immunofluorescent staining techniques for their patterns of intermediate filament (keratin, vimentin, and neurofilament triplet protein) expression. All cell lines examined, both small cell lung carcinoma (SCLC) and non-SCLC (squamous cell carcinoma, adenocarcinoma, large cell carcinoma, and mesothelioma) contained keratin, consistent with their epithelial derivation. These lung carcinoma cell lines also expressed vimentin, the characteristic intermediate filament of mesenchymal cells in vivo. In light of the proposed neuroectodermal origin of SCLC, cell lines were also studied for neurofilament expression. Two of four SCLC tumor cell lines, as well as non-SCLC cell lines, showed no reactivity with antibodies to neurofilament triplet protein. Two of the SCLC cell lines stained weakly with anti-neurofilament antibody. Examination of specific keratin patterns in human lung tumor cell lines by selective immunoprecipitation with keratin antiserum and sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that small-sized keratin proteins (M(r) 44,000 to 52,000) were present in cell lines derived from SCLC and non-SCLC types of lung cancer. Tumor cell lines exhibiting squamous differentiation by light microscopic criteria (i.e., intracellular keratin, intercellular bridging, 'pearl' formation, and/or individual cell keratinization) also displayed a preponderance of intermediate-sized keratins (M(r) 57,000 and 59,000) and exhibited another feature of terminal keratinocyte differentiation (cross-linked envelope formation). Mesothelioma cell lines had varying keratin profiles. The presence of keratin proteins in all SCLC cell lines examined argues against a neuroectodermal origin for these tumors and is consistent with the notion that these tumors arise from a common bronchial 'stem cell', similar to that from which other types of bronchogenic carcinomas arise.

Original languageEnglish (US)
Pages (from-to)1187-1197
Number of pages11
JournalCancer Research
Volume45
Issue number3
StatePublished - 1985

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Intermediate Filaments
Keratins
Tumor Cell Line
Small Cell Lung Carcinoma
Lung
Cell Line
Non-Small Cell Lung Carcinoma
Neurofilament Proteins
Mesothelioma
Vimentin
Lung Neoplasms
Neuroectodermal Tumors
Large Cell Carcinoma
Bronchogenic Carcinoma
Keratinocytes
Immunoprecipitation
Sodium Dodecyl Sulfate
Immune Sera
Polyacrylamide Gel Electrophoresis
Anti-Idiotypic Antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Banks-Schlegel, S. P., Gazdar, A. F., & Harris, C. C. (1985). Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Research, 45(3), 1187-1197.

Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. / Banks-Schlegel, S. P.; Gazdar, A. F.; Harris, C. C.

In: Cancer Research, Vol. 45, No. 3, 1985, p. 1187-1197.

Research output: Contribution to journalArticle

Banks-Schlegel, SP, Gazdar, AF & Harris, CC 1985, 'Intermediate filament and cross-linked envelope expression in human lung tumor cell lines', Cancer Research, vol. 45, no. 3, pp. 1187-1197.
Banks-Schlegel, S. P. ; Gazdar, A. F. ; Harris, C. C. / Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. In: Cancer Research. 1985 ; Vol. 45, No. 3. pp. 1187-1197.
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