International descriptive and interventional survey for oxycholesterol determination by gas- and liquid-chromatographic methods

Dieter Lütjohann, Ingemar Björkhem, Silvia Friedrichs, Anja Kerksiek, Wolf Jochen Geilenkeuser, Anita Lövgren-Sandblom, Diana Ansorena, Iciar Astiasarán, Lucía Baila-Rueda, Blanca Barriuso, Lionell Bretillon, Richard W. Browne, Claudio Caccia, Ana Cenarro, Peter J. Crick, Günter Fauler, Guadalupe García-Llatas, William J. Griffiths, Luigi Iuliano, María Jésus LagardaValerio Leoni, Ana Maria Lottenberg, Silke Matysik, Jeff McDonald, Todd C. Rideout, Gerd Schmitz, Valéria Sutti Nunes, Yuqin Wang, Chiara Zerbinati, Ulf Diczfalusy, Hans Frieder Schött

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Increasing numbers of laboratories develop new methods based on gas-liquid and high-performance liquid chromatography to determine serum concentrations of oxygenated cholesterol metabolites such as 7α-, 24(S)-, and 27-hydroxycholesterol. We initiated a first international descriptive oxycholesterol (OCS) survey in 2013 and a second interventional survey 2014 in order to compare levels of OCS reported by different laboratories and to define possible sources of analytical errors. In 2013 a set of two lyophilized serum pools (A and B) was sent to nine laboratories in different countries for OCS measurement utilizing their own standard stock solutions. In 2014 eleven laboratories were requested to determine OCS concentrations in lyophilized pooled sera (C and D) utilizing the same provided standard stock solutions of OCS. The participating laboratories submitted results obtained after capillary gas-liquid chromatography-mass selective detection with either epicoprostanol or deuterium labelled sterols as internal standards and high-performance liquid chromatography with mass selective detection and deuterated OCS as internal standard. Each participant received a clear overview of the results in form of Youden-Plots and basic statistical evaluation in its used unit. The coefficients of variation of the concentrations obtained by all laboratories using their individual methods were 58.5–73.3% (survey 1), 56.8–60.3% (survey 2); 36.2–35.8% (survey 1), 56.6–59.8, (survey 2); 61.1–197.7% (survey 1), 47.2–74.2% (survey 2) for 24(S)-, 27-, and 7α-hydroxycholesterol, respectively. We are surprised by the very great differences between the laboratories, even under conditions when the same standards were used. The values of OCS's must be evaluated in relation to the analytical technique used, the efficiency of the ample separation and the nature of the internal standard used. Quantification of the calibration solution and inappropriate internal standards could be identified as major causes for the high variance in the reported results from the different laboratories. A harmonisation of analytical standard methods is highly needed.

Original languageEnglish (US)
Pages (from-to)26-32
Number of pages7
JournalBiochimie
Volume153
DOIs
StatePublished - Oct 2018

Keywords

  • Bile acid precursors
  • Gas-liquid chromatography
  • High performance-liquid chromatography
  • Isotope dilution
  • Mass spectrometry
  • Oxysterol

ASJC Scopus subject areas

  • Biochemistry

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    Lütjohann, D., Björkhem, I., Friedrichs, S., Kerksiek, A., Geilenkeuser, W. J., Lövgren-Sandblom, A., Ansorena, D., Astiasarán, I., Baila-Rueda, L., Barriuso, B., Bretillon, L., Browne, R. W., Caccia, C., Cenarro, A., Crick, P. J., Fauler, G., García-Llatas, G., Griffiths, W. J., Iuliano, L., ... Schött, H. F. (2018). International descriptive and interventional survey for oxycholesterol determination by gas- and liquid-chromatographic methods. Biochimie, 153, 26-32. https://doi.org/10.1016/j.biochi.2018.07.016