Interphasezytogenetik mit DNA-sonden für chromosom 8 zur detektion zirkulierender tumorzellen beim mammakarzinom

Translated title of the contribution: Interphase cytogenetics with DNA-probes for chromosome 8 to detect circulating tumor cells in breast cancer patients

B. M. Ghadimi, J. Uhr, Th Tucker, K. Heselmeyer-Haddad, G. Auer, Th Ried, H. Becker

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The detection of micrometastases in the bone marrow or peripheral blood of cancer patients is increasingly used for a more sensitive tumor staging and prognostication. The potential value of the currently used techniques for the detection of epithelial antigens by RT-PCR or immunohistochemistry in respect of specificity is currently controversially discussed. In the present study we demonstrate a new approach which enables the direct visualization of the tumor specific alteration of chromosome 8 in circulating tumor cells. We have therefore studied breast cancer patients with various tumor stages and tried to determine the frequency of circulating tumor cells in the peripheral blood by using interphase cytogenetics for chromosome 7 and 8. Imprints of primary breast cancers and cytospins with circulating tumor cells of corresponding patients were studied in a blinded fashion. The blood samples were generated by immunomagnetic enrichment of circulating tumor cells from peripheral blood by ferrofluid and centrifugation onto cover slips. These cytospins were then hybridized with centromer probes 7 and 8. After analyzing 27 patients with benign as well as malignant breast tumors we can demonstrate that the chromosomal pattern between malignant tumor and corresponding circulating tumor cells is identical. Furthermore, the detection of circulating tumor cells directly correlates with the primary tumor stage. We did not find any cells with chromosome 8 alterations in the patients with benign disease. Surprisingly, even in early breast cancers (T1 NO) interphase cytogenetics identified circulating tumor cells in 2 out of 4 patients. In conclusion, interphase cytogenetics represent a non-invasive, sensitive and specific assay for the direct visualization of circulating tumor cells in the peripheral blood. The prognostic value of these findings remains to be further evaluated in larger prospective studies.

Original languageGerman
Pages (from-to)922-925
Number of pages4
JournalZentralblatt fur Chirurgie
Volume126
Issue number11
DOIs
StatePublished - 2001

Fingerprint

Circulating Neoplastic Cells
Chromosomes, Human, Pair 8
Interphase
DNA Probes
Cytogenetics
Breast Neoplasms
Neoplasms
Neoplasm Micrometastasis
Chromosomes, Human, Pair 7
Neoplasm Staging
Centrifugation
Bone Marrow
Immunohistochemistry
Prospective Studies
Antigens
Polymerase Chain Reaction

Keywords

  • Breast cancer
  • Chromosome 8
  • Interphase cytogenetics
  • Micrometastases
  • Tumorstaging

ASJC Scopus subject areas

  • Surgery

Cite this

Ghadimi, B. M., Uhr, J., Tucker, T., Heselmeyer-Haddad, K., Auer, G., Ried, T., & Becker, H. (2001). Interphasezytogenetik mit DNA-sonden für chromosom 8 zur detektion zirkulierender tumorzellen beim mammakarzinom. Zentralblatt fur Chirurgie, 126(11), 922-925. https://doi.org/10.1055/s-2001-19148

Interphasezytogenetik mit DNA-sonden für chromosom 8 zur detektion zirkulierender tumorzellen beim mammakarzinom. / Ghadimi, B. M.; Uhr, J.; Tucker, Th; Heselmeyer-Haddad, K.; Auer, G.; Ried, Th; Becker, H.

In: Zentralblatt fur Chirurgie, Vol. 126, No. 11, 2001, p. 922-925.

Research output: Contribution to journalArticle

Ghadimi, BM, Uhr, J, Tucker, T, Heselmeyer-Haddad, K, Auer, G, Ried, T & Becker, H 2001, 'Interphasezytogenetik mit DNA-sonden für chromosom 8 zur detektion zirkulierender tumorzellen beim mammakarzinom', Zentralblatt fur Chirurgie, vol. 126, no. 11, pp. 922-925. https://doi.org/10.1055/s-2001-19148
Ghadimi, B. M. ; Uhr, J. ; Tucker, Th ; Heselmeyer-Haddad, K. ; Auer, G. ; Ried, Th ; Becker, H. / Interphasezytogenetik mit DNA-sonden für chromosom 8 zur detektion zirkulierender tumorzellen beim mammakarzinom. In: Zentralblatt fur Chirurgie. 2001 ; Vol. 126, No. 11. pp. 922-925.
@article{436cb130a1224dab8ce55d90ad698ba5,
title = "Interphasezytogenetik mit DNA-sonden f{\"u}r chromosom 8 zur detektion zirkulierender tumorzellen beim mammakarzinom",
abstract = "The detection of micrometastases in the bone marrow or peripheral blood of cancer patients is increasingly used for a more sensitive tumor staging and prognostication. The potential value of the currently used techniques for the detection of epithelial antigens by RT-PCR or immunohistochemistry in respect of specificity is currently controversially discussed. In the present study we demonstrate a new approach which enables the direct visualization of the tumor specific alteration of chromosome 8 in circulating tumor cells. We have therefore studied breast cancer patients with various tumor stages and tried to determine the frequency of circulating tumor cells in the peripheral blood by using interphase cytogenetics for chromosome 7 and 8. Imprints of primary breast cancers and cytospins with circulating tumor cells of corresponding patients were studied in a blinded fashion. The blood samples were generated by immunomagnetic enrichment of circulating tumor cells from peripheral blood by ferrofluid and centrifugation onto cover slips. These cytospins were then hybridized with centromer probes 7 and 8. After analyzing 27 patients with benign as well as malignant breast tumors we can demonstrate that the chromosomal pattern between malignant tumor and corresponding circulating tumor cells is identical. Furthermore, the detection of circulating tumor cells directly correlates with the primary tumor stage. We did not find any cells with chromosome 8 alterations in the patients with benign disease. Surprisingly, even in early breast cancers (T1 NO) interphase cytogenetics identified circulating tumor cells in 2 out of 4 patients. In conclusion, interphase cytogenetics represent a non-invasive, sensitive and specific assay for the direct visualization of circulating tumor cells in the peripheral blood. The prognostic value of these findings remains to be further evaluated in larger prospective studies.",
keywords = "Breast cancer, Chromosome 8, Interphase cytogenetics, Micrometastases, Tumorstaging",
author = "Ghadimi, {B. M.} and J. Uhr and Th Tucker and K. Heselmeyer-Haddad and G. Auer and Th Ried and H. Becker",
year = "2001",
doi = "10.1055/s-2001-19148",
language = "German",
volume = "126",
pages = "922--925",
journal = "Zentralblatt f{\"u}r Chirurgie",
issn = "0044-409X",
publisher = "Georg Thieme Verlag",
number = "11",

}

TY - JOUR

T1 - Interphasezytogenetik mit DNA-sonden für chromosom 8 zur detektion zirkulierender tumorzellen beim mammakarzinom

AU - Ghadimi, B. M.

AU - Uhr, J.

AU - Tucker, Th

AU - Heselmeyer-Haddad, K.

AU - Auer, G.

AU - Ried, Th

AU - Becker, H.

PY - 2001

Y1 - 2001

N2 - The detection of micrometastases in the bone marrow or peripheral blood of cancer patients is increasingly used for a more sensitive tumor staging and prognostication. The potential value of the currently used techniques for the detection of epithelial antigens by RT-PCR or immunohistochemistry in respect of specificity is currently controversially discussed. In the present study we demonstrate a new approach which enables the direct visualization of the tumor specific alteration of chromosome 8 in circulating tumor cells. We have therefore studied breast cancer patients with various tumor stages and tried to determine the frequency of circulating tumor cells in the peripheral blood by using interphase cytogenetics for chromosome 7 and 8. Imprints of primary breast cancers and cytospins with circulating tumor cells of corresponding patients were studied in a blinded fashion. The blood samples were generated by immunomagnetic enrichment of circulating tumor cells from peripheral blood by ferrofluid and centrifugation onto cover slips. These cytospins were then hybridized with centromer probes 7 and 8. After analyzing 27 patients with benign as well as malignant breast tumors we can demonstrate that the chromosomal pattern between malignant tumor and corresponding circulating tumor cells is identical. Furthermore, the detection of circulating tumor cells directly correlates with the primary tumor stage. We did not find any cells with chromosome 8 alterations in the patients with benign disease. Surprisingly, even in early breast cancers (T1 NO) interphase cytogenetics identified circulating tumor cells in 2 out of 4 patients. In conclusion, interphase cytogenetics represent a non-invasive, sensitive and specific assay for the direct visualization of circulating tumor cells in the peripheral blood. The prognostic value of these findings remains to be further evaluated in larger prospective studies.

AB - The detection of micrometastases in the bone marrow or peripheral blood of cancer patients is increasingly used for a more sensitive tumor staging and prognostication. The potential value of the currently used techniques for the detection of epithelial antigens by RT-PCR or immunohistochemistry in respect of specificity is currently controversially discussed. In the present study we demonstrate a new approach which enables the direct visualization of the tumor specific alteration of chromosome 8 in circulating tumor cells. We have therefore studied breast cancer patients with various tumor stages and tried to determine the frequency of circulating tumor cells in the peripheral blood by using interphase cytogenetics for chromosome 7 and 8. Imprints of primary breast cancers and cytospins with circulating tumor cells of corresponding patients were studied in a blinded fashion. The blood samples were generated by immunomagnetic enrichment of circulating tumor cells from peripheral blood by ferrofluid and centrifugation onto cover slips. These cytospins were then hybridized with centromer probes 7 and 8. After analyzing 27 patients with benign as well as malignant breast tumors we can demonstrate that the chromosomal pattern between malignant tumor and corresponding circulating tumor cells is identical. Furthermore, the detection of circulating tumor cells directly correlates with the primary tumor stage. We did not find any cells with chromosome 8 alterations in the patients with benign disease. Surprisingly, even in early breast cancers (T1 NO) interphase cytogenetics identified circulating tumor cells in 2 out of 4 patients. In conclusion, interphase cytogenetics represent a non-invasive, sensitive and specific assay for the direct visualization of circulating tumor cells in the peripheral blood. The prognostic value of these findings remains to be further evaluated in larger prospective studies.

KW - Breast cancer

KW - Chromosome 8

KW - Interphase cytogenetics

KW - Micrometastases

KW - Tumorstaging

UR - http://www.scopus.com/inward/record.url?scp=0035204388&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035204388&partnerID=8YFLogxK

U2 - 10.1055/s-2001-19148

DO - 10.1055/s-2001-19148

M3 - Article

C2 - 11753805

AN - SCOPUS:0035204388

VL - 126

SP - 922

EP - 925

JO - Zentralblatt für Chirurgie

JF - Zentralblatt für Chirurgie

SN - 0044-409X

IS - 11

ER -