TY - GEN
T1 - Interphase fluorescence in situ hybridization signal detection by computing intensity variance along the optical axis
AU - Li, Zheng
AU - Zheng, Bin
AU - Ren, Liqiang
AU - Liu, Hong
PY - 2014
Y1 - 2014
N2 - Fluorescence in situ Hybridization technology is a commonly used tool to detect chromosome aberrations, which are often pathologically significant. Since manual FISH analysis is a tedious and time-consuming procedure, reliable and robust automated image acquisition and analysis are in demand. Under high magnification objective lenses such as 60x and 100x, the depth of field will often be too small and the FISH probes may not always lie in the same focal plane. A statistical variance based automated FISH analysis method is developed in order to address this problem. On a stack of slices at consecutive image planes with a step size d, the statistical variance alone the z-axis is calculated to form a 2-D matrix. Since pixels shift dramatically to high intensity at FISH probe location, the probes will manifest high peak values in the matrix. A computer-aided detection scheme based on top-hat transform is applied to the matrix to detect FISH probe signals. This study demonstrates a simple and robust method for FISH probe detection as well as a way of 2- D representation of 3-D data.
AB - Fluorescence in situ Hybridization technology is a commonly used tool to detect chromosome aberrations, which are often pathologically significant. Since manual FISH analysis is a tedious and time-consuming procedure, reliable and robust automated image acquisition and analysis are in demand. Under high magnification objective lenses such as 60x and 100x, the depth of field will often be too small and the FISH probes may not always lie in the same focal plane. A statistical variance based automated FISH analysis method is developed in order to address this problem. On a stack of slices at consecutive image planes with a step size d, the statistical variance alone the z-axis is calculated to form a 2-D matrix. Since pixels shift dramatically to high intensity at FISH probe location, the probes will manifest high peak values in the matrix. A computer-aided detection scheme based on top-hat transform is applied to the matrix to detect FISH probe signals. This study demonstrates a simple and robust method for FISH probe detection as well as a way of 2- D representation of 3-D data.
KW - Extended depth of field
KW - Fluorescence in situ hybridization
KW - Fluorescence microscopy
UR - http://www.scopus.com/inward/record.url?scp=84901793641&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901793641&partnerID=8YFLogxK
U2 - 10.1117/12.2041307
DO - 10.1117/12.2041307
M3 - Conference contribution
AN - SCOPUS:84901793641
SN - 9780819498571
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Biophotonics and Immune Responses IX
PB - SPIE
T2 - Biophotonics and Immune Responses IX
Y2 - 3 February 2014 through 3 February 2014
ER -