Interrogating cell division errors using random and chromosome-specific missegregation approaches

Peter Ly, Don W. Cleveland

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Accurate segregation of the duplicated genome in mitosis is essential for maintaining genetic stability. Errors in this process can cause numerical and/or structural chromosome abnormalities–hallmark genomic features commonly associated with both tumorigenesis and developmental disorders. A cell-based approach was recently developed permitting inducible missegregation of the human Y chromosome by selectively disrupting kinetochore assembly onto the Y centromere. Although this strategy initially requires several steps of genetic manipulation, it is easy to use, highly efficient and specific for the Y without affecting the autosomes or the X, and does not require cell cycle synchronization or mitotic perturbation. Here we describe currently available tools for studying chromosome segregation errors, aneuploidy, and micronuclei, as well as discuss how the Y-specific missegregation system has been used to elucidate how chromosomal micronucleation can trigger a class of extensive rearrangements termed chromothripsis. The combinatorial use of these different tools will allow unresolved aspects of cell division defects and chromosomal instability to be experimentally explored.

Original languageEnglish (US)
Pages (from-to)1252-1258
Number of pages7
JournalCell Cycle
Volume16
Issue number13
DOIs
StatePublished - Jul 3 2017
Externally publishedYes

Keywords

  • aneuploidy
  • centromere
  • chromosome segregation
  • chromothripsis
  • micronuclei
  • mitosis

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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