Intestinal Edema

Effect of Enteral Feeding on Motility and Gene Expression

Stacey D. Moore-Olufemi, Jeff Padalecki, Shodimu E. Olufemi, Hasen Xue, Dwight H. Oliver, Ravi S. Radhakrishnan, Steve J. Allen, Fred A. Moore, Randy Stewart, Glen A. Laine, Charles S. Cox

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: Edema formation, inflammation, and ileus in the intestine are commonly seen in conditions like gastroschisis, inflammatory bowel disease, and cirrhosis. We hypothesized that early enteral feeding would improve intestinal transit. We also wanted to study the impact of early enteral feeding on global gene expression in the intestine. Design: Rats were divided into Sham or Edema ± immediate enteral nutrition (IEN). At 12 h, small intestinal transit via FITC-Dextran and tissue water were measured. Ileum was harvested for total RNA to analyze gene expression using cDNA microarray with validation using real-time PCR. Data are expressed as mean ± SEM, n = 4-6 and *, ** = P < 0.05 versus all groups using ANOVA. Results: IEN markedly improved intestinal transit with minimal genetic alterations in Edema animals. Major alterations in gene expression were detected in primary, cellular and macromolecular metabolic activities. Edema also altered more genes involved with the regulation of the actin cytoskeleton. Conclusions: Intestinal edema results in impaired small intestinal transit and globally increased gene expression. Early enteral nutrition improves edema-induced impaired transit and minimizes gene transcriptional activity.

Original languageEnglish (US)
Pages (from-to)283-292
Number of pages10
JournalJournal of Surgical Research
Volume155
Issue number2
DOIs
StatePublished - Aug 2009

Fingerprint

Enteral Nutrition
Edema
Gene Expression
Intestines
Gastroschisis
Ileus
Oligonucleotide Array Sequence Analysis
Actin Cytoskeleton
Ileum
Inflammatory Bowel Diseases
Genes
Real-Time Polymerase Chain Reaction
Analysis of Variance
Fibrosis
RNA
Inflammation
Water

Keywords

  • enteral feeding
  • ileus
  • intestinal edema
  • micorarray

ASJC Scopus subject areas

  • Surgery

Cite this

Moore-Olufemi, S. D., Padalecki, J., Olufemi, S. E., Xue, H., Oliver, D. H., Radhakrishnan, R. S., ... Cox, C. S. (2009). Intestinal Edema: Effect of Enteral Feeding on Motility and Gene Expression. Journal of Surgical Research, 155(2), 283-292. https://doi.org/10.1016/j.jss.2008.08.040

Intestinal Edema : Effect of Enteral Feeding on Motility and Gene Expression. / Moore-Olufemi, Stacey D.; Padalecki, Jeff; Olufemi, Shodimu E.; Xue, Hasen; Oliver, Dwight H.; Radhakrishnan, Ravi S.; Allen, Steve J.; Moore, Fred A.; Stewart, Randy; Laine, Glen A.; Cox, Charles S.

In: Journal of Surgical Research, Vol. 155, No. 2, 08.2009, p. 283-292.

Research output: Contribution to journalArticle

Moore-Olufemi, SD, Padalecki, J, Olufemi, SE, Xue, H, Oliver, DH, Radhakrishnan, RS, Allen, SJ, Moore, FA, Stewart, R, Laine, GA & Cox, CS 2009, 'Intestinal Edema: Effect of Enteral Feeding on Motility and Gene Expression', Journal of Surgical Research, vol. 155, no. 2, pp. 283-292. https://doi.org/10.1016/j.jss.2008.08.040
Moore-Olufemi, Stacey D. ; Padalecki, Jeff ; Olufemi, Shodimu E. ; Xue, Hasen ; Oliver, Dwight H. ; Radhakrishnan, Ravi S. ; Allen, Steve J. ; Moore, Fred A. ; Stewart, Randy ; Laine, Glen A. ; Cox, Charles S. / Intestinal Edema : Effect of Enteral Feeding on Motility and Gene Expression. In: Journal of Surgical Research. 2009 ; Vol. 155, No. 2. pp. 283-292.
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