Intestinal microbiota promote enteric virus replication and systemic pathogenesis

Sharon K. Kuss, Gavin T. Best, Chris A. Etheredge, Andrea J. Pruijssers, Johnna M. Frierson, Lora V. Hooper, Terence S. Dermody, Julie K. Pfeiffer

Research output: Contribution to journalArticle

267 Citations (Scopus)

Abstract

Intestinal bacteria aid host health and limit bacterial pathogen colonization. However, the influence of bacteria on enteric viruses is largely unknown. We depleted the intestinal microbiota of mice with antibiotics before inoculation with poliovirus, an enteric virus. Antibiotic-treated mice were less susceptible to poliovirus disease and supported minimal viral replication in the intestine. Exposure to bacteria or their N-acetylglucosamine-containing surface polysaccharides, including lipopolysaccharide and peptidoglycan, enhanced poliovirus infectivity. We found that poliovirus binds lipopolysaccharide, and exposure of poliovirus to bacteria enhanced host cell association and infection. The pathogenesis of reovirus, an unrelated enteric virus, also was more severe in the presence of intestinal microbes. These results suggest that antibiotic-mediated microbiota depletion diminishes enteric virus infection and that enteric viruses exploit intestinal microbes for replication and transmission.

Original languageEnglish (US)
Pages (from-to)249-252
Number of pages4
JournalScience
Volume334
Issue number6053
DOIs
StatePublished - Oct 14 2011

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Poliovirus
Enterovirus
Virus Replication
Bacteria
Anti-Bacterial Agents
Lipopolysaccharides
Enterovirus Infections
Acetylglucosamine
Peptidoglycan
Microbiota
Intestines
Polysaccharides
Gastrointestinal Microbiome
Health
Infection

ASJC Scopus subject areas

  • General

Cite this

Kuss, S. K., Best, G. T., Etheredge, C. A., Pruijssers, A. J., Frierson, J. M., Hooper, L. V., ... Pfeiffer, J. K. (2011). Intestinal microbiota promote enteric virus replication and systemic pathogenesis. Science, 334(6053), 249-252. https://doi.org/10.1126/science.1211057

Intestinal microbiota promote enteric virus replication and systemic pathogenesis. / Kuss, Sharon K.; Best, Gavin T.; Etheredge, Chris A.; Pruijssers, Andrea J.; Frierson, Johnna M.; Hooper, Lora V.; Dermody, Terence S.; Pfeiffer, Julie K.

In: Science, Vol. 334, No. 6053, 14.10.2011, p. 249-252.

Research output: Contribution to journalArticle

Kuss, SK, Best, GT, Etheredge, CA, Pruijssers, AJ, Frierson, JM, Hooper, LV, Dermody, TS & Pfeiffer, JK 2011, 'Intestinal microbiota promote enteric virus replication and systemic pathogenesis', Science, vol. 334, no. 6053, pp. 249-252. https://doi.org/10.1126/science.1211057
Kuss SK, Best GT, Etheredge CA, Pruijssers AJ, Frierson JM, Hooper LV et al. Intestinal microbiota promote enteric virus replication and systemic pathogenesis. Science. 2011 Oct 14;334(6053):249-252. https://doi.org/10.1126/science.1211057
Kuss, Sharon K. ; Best, Gavin T. ; Etheredge, Chris A. ; Pruijssers, Andrea J. ; Frierson, Johnna M. ; Hooper, Lora V. ; Dermody, Terence S. ; Pfeiffer, Julie K. / Intestinal microbiota promote enteric virus replication and systemic pathogenesis. In: Science. 2011 ; Vol. 334, No. 6053. pp. 249-252.
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