Intra-tracheal administration of Haemophilus influenzae in mouse models to study airway inflammation

K. Venuprasad, Balamayooran Theivanthiran, Brandi Cantarel

Research output: Contribution to journalArticle

Abstract

Here, we describe a detailed procedure to efficiently and directly deliver Haemophilus influenzae into the lower respiratory tracts of mice. We demonstrate the procedure for preparing H. influenzae inoculum, intra-tracheal instillation of H. influenzae into the lung, collection of broncho-alveolar lavage fluid (BALF), analysis of immune cells in the BALF, and RNA isolation for differential gene expression analysis. This procedure can be used to study the lung inflammatory response to any bacteria, virus or fungi. Direct tracheal instillation is mostly preferred over intranasal or aerosol inhalation procedures because it more efficiently delivers the bacterial inoculum into the lower respiratory tract with less ambiguity.

Original languageEnglish (US)
Article numbere53964
JournalJournal of Visualized Experiments
Volume2016
Issue number109
DOIs
StatePublished - Mar 2 2016

Keywords

  • Cell signalling
  • Gene expression
  • Haemophilus influenzae
  • Immunology
  • Inflammation
  • Intra-tracheal
  • Issue 109
  • Respiratory pathogen

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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