Intracellular free calcium increases in cultured cortical neurons deprived of oxygen and glucose.

M. P. Goldberg, D. W. Choi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Dissociated neocortical cultures from fetal mice exposed transiently to a medium lacking both glucose and oxygen developed neuronal degeneration without glial degeneration. We have found that this injury depends on extracellular calcium and is associated with uptake of calcium from the culture medium. We measured free cytoplasmic calcium in individual neurons using the fluorescent calcium indicator fluo-3 and provide evidence that oxygen and glucose deprivation injury increases the intracellular calcium signal. Both intracellular calcium elevation and subsequent neuronal loss could be blocked by the N-methyl-D-aspartate receptor antagonist dextrorphan.

Original languageEnglish (US)
JournalStroke
Volume21
Issue number11 Suppl
StatePublished - Nov 1990

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Oxygen
Calcium
Neurons
Glucose
Dextrorphan
Wounds and Injuries
N-Methyl-D-Aspartate Receptors
Neuroglia
Culture Media

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Intracellular free calcium increases in cultured cortical neurons deprived of oxygen and glucose. / Goldberg, M. P.; Choi, D. W.

In: Stroke, Vol. 21, No. 11 Suppl, 11.1990.

Research output: Contribution to journalArticle

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