Intracellular free calcium increases in cultured cortical neurons deprived of oxygen and glucose.

M. P. Goldberg, D. W. Choi

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Dissociated neocortical cultures from fetal mice exposed transiently to a medium lacking both glucose and oxygen developed neuronal degeneration without glial degeneration. We have found that this injury depends on extracellular calcium and is associated with uptake of calcium from the culture medium. We measured free cytoplasmic calcium in individual neurons using the fluorescent calcium indicator fluo-3 and provide evidence that oxygen and glucose deprivation injury increases the intracellular calcium signal. Both intracellular calcium elevation and subsequent neuronal loss could be blocked by the N-methyl-D-aspartate receptor antagonist dextrorphan.

Original languageEnglish (US)
Pages (from-to)III75-77
JournalStroke
Volume21
Issue number11 Suppl
StatePublished - Nov 1990

ASJC Scopus subject areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

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