Intracoronary shunt-induced endothelial cell damage in porcine heart

James H. Dygert, Hemant S. Thatte, Dharam J. Kumbhani, Samer F. Najjar, Patrick R. Treanor, Shukri F. Khuri

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background. Injury to and dysfunction of the endothelium can compromise the patency of coronary arteries and lead to impaired perfusion of the heart. Such injury can occur after the application of an intravascular shunt and/or a snare to a coronary artery during bypass surgery. In this study, multiphoton microscopy was used to assess the integrity of endothelium in porcine coronary arteries subjected to shunting and snaring during off-pump coronary artery bypass grafting (OPCAB). Materials and methods. In open chest porcine, the left anterior descending artery was manipulated in different regions using snare and shunt to simulate OPCAB. Sections of left anterior descending artery were labeled with fluorescent dyes to evaluate the viability of the endothelium. The structural integrity of the endothelium was evaluated by calcein- and ethidium homodimer-mediated fluorescence. Endothelial functional viability was assessed by measuring cellular esterase activity, calcium mobilization, and endothelial nitric oxide synthase-mediated generation of nitric oxide using fluorescence dyes and multiphoton microscopy. Results. Substantial endothelial damage was observed in shunted region of the coronary arteries. In contrast, endothelium remained structurally viable in regions that were snared, similar to control regions of the coronary arteries that were not manipulated. Esterase activity, calcium mobilization, and nitric oxide generation was greater in the control and snared regions of the coronary arteries in comparison to the shunted region. Conclusions. The use of intracoronary shunts led to structural damage and attenuation of endothelial function in porcine coronary arteries, whereas snared vessels maintained their viability and integrity, similar to the control sections. The routine use of shunts in OPCABG may lead to endothelial damage and possibly to long-term graft failure.

Original languageEnglish (US)
Pages (from-to)168-174
Number of pages7
JournalJournal of Surgical Research
Volume131
Issue number2
DOIs
StatePublished - Apr 2006

Fingerprint

Coronary Vessels
Swine
Endothelial Cells
Endothelium
Coronary Artery Bypass
Off-Pump Coronary Artery Bypass
Esterases
Multiphoton Fluorescence Microscopy
Nitric Oxide
Arteries
Nitric Oxide Synthase Type III
Wounds and Injuries
Fluorescent Dyes
Microscopy
Coloring Agents
Thorax
Perfusion
Fluorescence
Calcium
Transplants

Keywords

  • Coronary artery
  • Endothelium
  • Nitric oxide
  • Off-pump bypass surgery

ASJC Scopus subject areas

  • Surgery

Cite this

Dygert, J. H., Thatte, H. S., Kumbhani, D. J., Najjar, S. F., Treanor, P. R., & Khuri, S. F. (2006). Intracoronary shunt-induced endothelial cell damage in porcine heart. Journal of Surgical Research, 131(2), 168-174. https://doi.org/10.1016/j.jss.2005.10.026

Intracoronary shunt-induced endothelial cell damage in porcine heart. / Dygert, James H.; Thatte, Hemant S.; Kumbhani, Dharam J.; Najjar, Samer F.; Treanor, Patrick R.; Khuri, Shukri F.

In: Journal of Surgical Research, Vol. 131, No. 2, 04.2006, p. 168-174.

Research output: Contribution to journalArticle

Dygert, JH, Thatte, HS, Kumbhani, DJ, Najjar, SF, Treanor, PR & Khuri, SF 2006, 'Intracoronary shunt-induced endothelial cell damage in porcine heart', Journal of Surgical Research, vol. 131, no. 2, pp. 168-174. https://doi.org/10.1016/j.jss.2005.10.026
Dygert, James H. ; Thatte, Hemant S. ; Kumbhani, Dharam J. ; Najjar, Samer F. ; Treanor, Patrick R. ; Khuri, Shukri F. / Intracoronary shunt-induced endothelial cell damage in porcine heart. In: Journal of Surgical Research. 2006 ; Vol. 131, No. 2. pp. 168-174.
@article{7c9784353cad44bba0b76fa699be5cb7,
title = "Intracoronary shunt-induced endothelial cell damage in porcine heart",
abstract = "Background. Injury to and dysfunction of the endothelium can compromise the patency of coronary arteries and lead to impaired perfusion of the heart. Such injury can occur after the application of an intravascular shunt and/or a snare to a coronary artery during bypass surgery. In this study, multiphoton microscopy was used to assess the integrity of endothelium in porcine coronary arteries subjected to shunting and snaring during off-pump coronary artery bypass grafting (OPCAB). Materials and methods. In open chest porcine, the left anterior descending artery was manipulated in different regions using snare and shunt to simulate OPCAB. Sections of left anterior descending artery were labeled with fluorescent dyes to evaluate the viability of the endothelium. The structural integrity of the endothelium was evaluated by calcein- and ethidium homodimer-mediated fluorescence. Endothelial functional viability was assessed by measuring cellular esterase activity, calcium mobilization, and endothelial nitric oxide synthase-mediated generation of nitric oxide using fluorescence dyes and multiphoton microscopy. Results. Substantial endothelial damage was observed in shunted region of the coronary arteries. In contrast, endothelium remained structurally viable in regions that were snared, similar to control regions of the coronary arteries that were not manipulated. Esterase activity, calcium mobilization, and nitric oxide generation was greater in the control and snared regions of the coronary arteries in comparison to the shunted region. Conclusions. The use of intracoronary shunts led to structural damage and attenuation of endothelial function in porcine coronary arteries, whereas snared vessels maintained their viability and integrity, similar to the control sections. The routine use of shunts in OPCABG may lead to endothelial damage and possibly to long-term graft failure.",
keywords = "Coronary artery, Endothelium, Nitric oxide, Off-pump bypass surgery",
author = "Dygert, {James H.} and Thatte, {Hemant S.} and Kumbhani, {Dharam J.} and Najjar, {Samer F.} and Treanor, {Patrick R.} and Khuri, {Shukri F.}",
year = "2006",
month = "4",
doi = "10.1016/j.jss.2005.10.026",
language = "English (US)",
volume = "131",
pages = "168--174",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Intracoronary shunt-induced endothelial cell damage in porcine heart

AU - Dygert, James H.

AU - Thatte, Hemant S.

AU - Kumbhani, Dharam J.

AU - Najjar, Samer F.

AU - Treanor, Patrick R.

AU - Khuri, Shukri F.

PY - 2006/4

Y1 - 2006/4

N2 - Background. Injury to and dysfunction of the endothelium can compromise the patency of coronary arteries and lead to impaired perfusion of the heart. Such injury can occur after the application of an intravascular shunt and/or a snare to a coronary artery during bypass surgery. In this study, multiphoton microscopy was used to assess the integrity of endothelium in porcine coronary arteries subjected to shunting and snaring during off-pump coronary artery bypass grafting (OPCAB). Materials and methods. In open chest porcine, the left anterior descending artery was manipulated in different regions using snare and shunt to simulate OPCAB. Sections of left anterior descending artery were labeled with fluorescent dyes to evaluate the viability of the endothelium. The structural integrity of the endothelium was evaluated by calcein- and ethidium homodimer-mediated fluorescence. Endothelial functional viability was assessed by measuring cellular esterase activity, calcium mobilization, and endothelial nitric oxide synthase-mediated generation of nitric oxide using fluorescence dyes and multiphoton microscopy. Results. Substantial endothelial damage was observed in shunted region of the coronary arteries. In contrast, endothelium remained structurally viable in regions that were snared, similar to control regions of the coronary arteries that were not manipulated. Esterase activity, calcium mobilization, and nitric oxide generation was greater in the control and snared regions of the coronary arteries in comparison to the shunted region. Conclusions. The use of intracoronary shunts led to structural damage and attenuation of endothelial function in porcine coronary arteries, whereas snared vessels maintained their viability and integrity, similar to the control sections. The routine use of shunts in OPCABG may lead to endothelial damage and possibly to long-term graft failure.

AB - Background. Injury to and dysfunction of the endothelium can compromise the patency of coronary arteries and lead to impaired perfusion of the heart. Such injury can occur after the application of an intravascular shunt and/or a snare to a coronary artery during bypass surgery. In this study, multiphoton microscopy was used to assess the integrity of endothelium in porcine coronary arteries subjected to shunting and snaring during off-pump coronary artery bypass grafting (OPCAB). Materials and methods. In open chest porcine, the left anterior descending artery was manipulated in different regions using snare and shunt to simulate OPCAB. Sections of left anterior descending artery were labeled with fluorescent dyes to evaluate the viability of the endothelium. The structural integrity of the endothelium was evaluated by calcein- and ethidium homodimer-mediated fluorescence. Endothelial functional viability was assessed by measuring cellular esterase activity, calcium mobilization, and endothelial nitric oxide synthase-mediated generation of nitric oxide using fluorescence dyes and multiphoton microscopy. Results. Substantial endothelial damage was observed in shunted region of the coronary arteries. In contrast, endothelium remained structurally viable in regions that were snared, similar to control regions of the coronary arteries that were not manipulated. Esterase activity, calcium mobilization, and nitric oxide generation was greater in the control and snared regions of the coronary arteries in comparison to the shunted region. Conclusions. The use of intracoronary shunts led to structural damage and attenuation of endothelial function in porcine coronary arteries, whereas snared vessels maintained their viability and integrity, similar to the control sections. The routine use of shunts in OPCABG may lead to endothelial damage and possibly to long-term graft failure.

KW - Coronary artery

KW - Endothelium

KW - Nitric oxide

KW - Off-pump bypass surgery

UR - http://www.scopus.com/inward/record.url?scp=33644900722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644900722&partnerID=8YFLogxK

U2 - 10.1016/j.jss.2005.10.026

DO - 10.1016/j.jss.2005.10.026

M3 - Article

C2 - 16412472

AN - SCOPUS:33644900722

VL - 131

SP - 168

EP - 174

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 2

ER -