Intradermal active full-length DNA Aβ42 immunization via electroporation leads to high anti-Aβ antibody levels in wild-type mice

Research output: Contribution to journalArticle

Abstract

Aβ immunotherapies with anti-Aβ antibody responses have high potential as possible prevention treatment for Alzheimer's disease. We have previously shown that active DNA Aβ1–42 immunization via gene gun delivery led to a non-inflammatory immune response resulting in decreased Aβ levels in brains of an immunized AD mouse model. To make DNA vaccination more applicable for clinical use, we used here intradermal electroporation. With fine tuning of the electropulse parameters, high antibody levels and low levels of inflammatory cytokines in the cellular immunoassays were observed. Full-length DNA Aβ1–42 immunization delivered via electroporation has potential to be used in the clinical setting.

Original languageEnglish (US)
Pages (from-to)15-25
Number of pages11
JournalJournal of Neuroimmunology
Volume322
DOIs
StatePublished - Sep 15 2018

Keywords

  • Alzheimer's disease
  • DNA Aβ42 immunotherapy
  • DNA vaccination
  • Electroporation
  • IgG isotypes
  • Immune response
  • Intradermal immunization
  • Non-inflammatory
  • Pulse parameter

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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