Intradermal active full-length DNA Aβ42 immunization via electroporation leads to high anti-Aβ antibody levels in wild-type mice

Research output: Contribution to journalArticle

Abstract

Aβ immunotherapies with anti-Aβ antibody responses have high potential as possible prevention treatment for Alzheimer's disease. We have previously shown that active DNA Aβ1–42 immunization via gene gun delivery led to a non-inflammatory immune response resulting in decreased Aβ levels in brains of an immunized AD mouse model. To make DNA vaccination more applicable for clinical use, we used here intradermal electroporation. With fine tuning of the electropulse parameters, high antibody levels and low levels of inflammatory cytokines in the cellular immunoassays were observed. Full-length DNA Aβ1–42 immunization delivered via electroporation has potential to be used in the clinical setting.

Original languageEnglish (US)
Pages (from-to)15-25
Number of pages11
JournalJournal of Neuroimmunology
Volume322
DOIs
StatePublished - Sep 15 2018

Fingerprint

Electroporation
Anti-Idiotypic Antibodies
Immunization
DNA
Firearms
Immunoassay
Immunotherapy
Antibody Formation
Alzheimer Disease
Vaccination
Cytokines
Antibodies
Brain
Genes
Therapeutics

Keywords

  • Alzheimer's disease
  • DNA Aβ42 immunotherapy
  • DNA vaccination
  • Electroporation
  • IgG isotypes
  • Immune response
  • Intradermal immunization
  • Non-inflammatory
  • Pulse parameter

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

@article{f33b27d58d2a477e9f3fc7d55e6fffcd,
title = "Intradermal active full-length DNA Aβ42 immunization via electroporation leads to high anti-Aβ antibody levels in wild-type mice",
abstract = "Aβ immunotherapies with anti-Aβ antibody responses have high potential as possible prevention treatment for Alzheimer's disease. We have previously shown that active DNA Aβ1–42 immunization via gene gun delivery led to a non-inflammatory immune response resulting in decreased Aβ levels in brains of an immunized AD mouse model. To make DNA vaccination more applicable for clinical use, we used here intradermal electroporation. With fine tuning of the electropulse parameters, high antibody levels and low levels of inflammatory cytokines in the cellular immunoassays were observed. Full-length DNA Aβ1–42 immunization delivered via electroporation has potential to be used in the clinical setting.",
keywords = "Alzheimer's disease, DNA Aβ42 immunotherapy, DNA vaccination, Electroporation, IgG isotypes, Immune response, Intradermal immunization, Non-inflammatory, Pulse parameter",
author = "Rosenberg, {Roger N.} and Min Fu and Doris Lambracht-Washington",
year = "2018",
month = "9",
day = "15",
doi = "10.1016/j.jneuroim.2018.05.017",
language = "English (US)",
volume = "322",
pages = "15--25",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier",

}

TY - JOUR

T1 - Intradermal active full-length DNA Aβ42 immunization via electroporation leads to high anti-Aβ antibody levels in wild-type mice

AU - Rosenberg, Roger N.

AU - Fu, Min

AU - Lambracht-Washington, Doris

PY - 2018/9/15

Y1 - 2018/9/15

N2 - Aβ immunotherapies with anti-Aβ antibody responses have high potential as possible prevention treatment for Alzheimer's disease. We have previously shown that active DNA Aβ1–42 immunization via gene gun delivery led to a non-inflammatory immune response resulting in decreased Aβ levels in brains of an immunized AD mouse model. To make DNA vaccination more applicable for clinical use, we used here intradermal electroporation. With fine tuning of the electropulse parameters, high antibody levels and low levels of inflammatory cytokines in the cellular immunoassays were observed. Full-length DNA Aβ1–42 immunization delivered via electroporation has potential to be used in the clinical setting.

AB - Aβ immunotherapies with anti-Aβ antibody responses have high potential as possible prevention treatment for Alzheimer's disease. We have previously shown that active DNA Aβ1–42 immunization via gene gun delivery led to a non-inflammatory immune response resulting in decreased Aβ levels in brains of an immunized AD mouse model. To make DNA vaccination more applicable for clinical use, we used here intradermal electroporation. With fine tuning of the electropulse parameters, high antibody levels and low levels of inflammatory cytokines in the cellular immunoassays were observed. Full-length DNA Aβ1–42 immunization delivered via electroporation has potential to be used in the clinical setting.

KW - Alzheimer's disease

KW - DNA Aβ42 immunotherapy

KW - DNA vaccination

KW - Electroporation

KW - IgG isotypes

KW - Immune response

KW - Intradermal immunization

KW - Non-inflammatory

KW - Pulse parameter

UR - http://www.scopus.com/inward/record.url?scp=85049028585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049028585&partnerID=8YFLogxK

U2 - 10.1016/j.jneuroim.2018.05.017

DO - 10.1016/j.jneuroim.2018.05.017

M3 - Article

C2 - 29958693

AN - SCOPUS:85049028585

VL - 322

SP - 15

EP - 25

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

ER -