Inverse relationship between constitutive γ-interferon production and human T-cell lymphoma/leukemia virus expression in cultured T lymphocytes

J. L. Moore, B. J. Poiesz, K. W. Zamkoff, S. A. Merl, S. Hanna, A. F. Gazdar, R. L. Comis

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Abstract

Particular interest in human T lymphocyte lymphoma/leukemia virus (HTLV) derives from the close association of HTLV with several types of human mature T lymphocyte malignancies and the strong possibility that HTLV is the causative agent of this group of leukemias and lymphomas. This is the first report to show that HTLV expression in T lymphocytes cultured in vitro is inversely proportional to constitutive γ interferon production. Of 16 fresh T lymphocyte cultures established from patients with mature T lymphocyte neoplasias, 3 were grown continuously for over 3 years and 13 were grown for 2 to 8 months in culture. Of the 16 cultures, 9 were HTLVp19 positive and interferon negative, whereas the remaining 7 were HTLVp19 negative or weakly positive and also interferon positive (12 to 105 U/ml). The prototype HTLV-positive T-cell line (HUT102) was examined over a long-term culture and after selective cell cloning for high virus yield. Results indicate that early-passage, low-HTLV-producing HUT102 cells constitutively produced significant levels of γ-immune interferon. In late-passage and cloned HUT102 cells, an increase in HTLV production was concordant with a decrease in constitutive interferon production and the loss of mature T lymphocyte antigens. Transformation of human umbilical cord blood lymphocytes by HTLV was possible only after cocultivation with the non-interferon, high virus-producing, cloned HUT102 T lymphocytes. The inverse relationship between interferon and HTLV production was also observed when normal human umbilical cord blood and adult T lymphocytes were transformed by HTLV and maintained in culture.

Original languageEnglish (US)
Pages (from-to)440-446
Number of pages7
JournalJournal of Virology
Volume53
Issue number2
StatePublished - 1985

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Deltaretrovirus
interferons
Interferons
T-lymphocytes
T-Lymphocytes
lymphoma
leukemia
Lymphoma
Leukemia
Viruses
viruses
umbilical cord
Fetal Blood

ASJC Scopus subject areas

  • Immunology

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Inverse relationship between constitutive γ-interferon production and human T-cell lymphoma/leukemia virus expression in cultured T lymphocytes. / Moore, J. L.; Poiesz, B. J.; Zamkoff, K. W.; Merl, S. A.; Hanna, S.; Gazdar, A. F.; Comis, R. L.

In: Journal of Virology, Vol. 53, No. 2, 1985, p. 440-446.

Research output: Contribution to journalArticle

Moore, J. L. ; Poiesz, B. J. ; Zamkoff, K. W. ; Merl, S. A. ; Hanna, S. ; Gazdar, A. F. ; Comis, R. L. / Inverse relationship between constitutive γ-interferon production and human T-cell lymphoma/leukemia virus expression in cultured T lymphocytes. In: Journal of Virology. 1985 ; Vol. 53, No. 2. pp. 440-446.
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