Investigation of low amyloid level toxicity effects on the function of hippocampal neurons

Elena Popugaeva, Ekaterina Pchitskaya, Hua Zhang, Olga Vlasova, Ilya Bezprozvanny

Research output: Contribution to journalArticle

Abstract

Alzheimer disease (AD) is responsible for the majority of elderly dementia cases. There are multiple mouse models of AD, however, none of them completely recapitulate human pathology. Moreover, it is even more difficult to imitate the onset of the disease in experimental models. In the current study we analyzed the influence of low amyloid level toxicity on the function of hippocampal neurons. Our assay is based on use of synthetic oligomeric amyloid beta peptides. These peptides were added to primary hippocampal cultures at a physiological nanomolar range to mimic early AD. Calcium imaging was used to evaluate the functionality of the assay. A low amyloid level toxicity assay could be useful for fundamental research as well as for testing newly developed AD drugs.

Original languageEnglish (US)
Pages (from-to)433-436
Number of pages4
JournalOpen Life Sciences
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2015

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Keywords

  • Alzheimer disease
  • amyloid beta
  • calcium imaging

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

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