Abstract
Background: A metabolomic study of biomarkers associated with dimethylnitrosamine (DMN)-induced hepatic fibrosis in Sprague-Dawley rats was performed using GC-MS. The clinical chemistry of the collected blood and the histopathology of excised liver samples were examined, and urine samples were prepared by solvent extraction. Results: Through pattern analysis, the DMN-treated group was divided into two subgroups based on the aspartate aminotransferase (AST) levels compared with the control, a moderately higher group (DMN subgroup A) and a significantly higher group (DMN subgroup B). Uric acid, orotic acid, N-phenylacetylglycine and glutaric acid were biomarkers for DMN subgroup A, aminomalonic acid was a biomarker for DMN subgroup B, and arabitol level distinguished control versus DMN treatment regardless of AST level. Conclusion: This study suggests that the identification and profiling of AST level-related metabolites may be useful as a diagnostic tool and for the study of the mechanism of liver fibrosis induced by DMN.
Original language | English (US) |
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Pages (from-to) | 41-51 |
Number of pages | 11 |
Journal | Bioanalysis |
Volume | 5 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2013 |
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmacology, Toxicology and Pharmaceutics(all)
- Clinical Biochemistry
- Medical Laboratory Technology