Abstract
Purpose: This study detects SARS-CoV-2 in the ocular surface through one-step reversetranscription droplet digital PCR (one-step RT-ddPCR) and evaluates the possibility of the ocular surface as a possible transmission route. Methods: A single-center prospective observational study was designed to investigate the viral loads in ocular surface. Specimens including the conjunctival swabs, nasopharyngeal swabs and blood were synchronously collected at a single time point for all COVID-19 patients. SARS-CoV-2 loads in nasopharyngeal swabs were tested by real-time polymerase chain reaction (PCR); the blood samples and conjunctival swabs were tested by real-time PCR and one-step RT-ddPCR. Results: Sixty-eight COVID-19 patients confirmed by nasopharyngeal real-time PCR were recruited. In the single time point test, 40 cases showed positive SARS-CoV-2 detection in either the blood, tears, or nasopharynx, of which four cases were triple-positive, 10 were dual-positive, and 26 were single-positive. The positive rate of nasopharyngeal swab realtime PCR test was 22.1% (15/68). The positive rate of blood and conjunctival swabs by onestep RT-ddPCR was 38.2% (26/68) and 25% (17/68), respectively, whereas real-time PCR was all negative. Positive conjunctival swabs were significantly correlated with positive nasopharyngeal swabs (P = 0.028). The sampling lags from illness onset to sampling day in 3 out of 4 triple-positive patients and in 9 out of 10 dual-positive patients were respectively less than 9 days and less than 20 days. Conclusion: Our results indicate that the positive rate of SARS-CoV-2 on the ocular surface is much higher than expected. Transmission possibility through the ocular surface may be greatly underestimated.
Original language | English (US) |
---|---|
Pages (from-to) | 5395-5401 |
Number of pages | 7 |
Journal | Infection and Drug Resistance |
Volume | 14 |
DOIs | |
State | Published - 2021 |
Externally published | Yes |
Keywords
- Absolute quantification
- Blood samples
- Nasopharyngeal specimens
- Tear samples
- Transmission
- Virus detection
ASJC Scopus subject areas
- Pharmacology
- Infectious Diseases
- Pharmacology (medical)