Involvement of CENP-F in histone methylation

Juan Du, Yan Li, Xueliang Zhu

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

CENP-F (also named mitosin) is a multifunctional protein of 350 kDa. In interphase, it is a nuclear protein, whereas in M phase it localizes to the kinetochore, the major microtubule-binding structure on chromosomes essential for chromosome segregation. CENP-F is also critical for myocyte differentiation through the interaction with Rb. It binds to ATF4 and negatively regulates the transcriptional activity of ATF4. It is also important for mitotic progression. Here we show that depletion of CENP-F by RNAi markedly downregulated the methylation of histone H3 at K4 and K9. Consistently, association of HP1 with mitotic chromosomes was largely decreased. These results uncover a novel role of CENP-F in regulation of epigenetic modification on histone H3.

Original languageEnglish (US)
Pages (from-to)173-176
Number of pages4
JournalActa Biochimica et Biophysica Sinica
Volume42
Issue number3
DOIs
StatePublished - Mar 2010
Externally publishedYes

Keywords

  • CENP-F
  • HP1
  • Histone
  • Methylation
  • Mitosin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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