Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine

Maria Lindskog, Per Svenningsson, Laura Pozzi, Yong Kim, Allen A. Flenberg, James A. Bibb, Bertil B. Fredholm, Angus C. Nairn, Paul Greengard, Gilberto Fisone

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Abstract

Caffeine has been imbibed since ancient times in tea and coffee, and more recently in colas. Caffeine owes its psychostimulant action to a blockade of adenosine A2A receptors, but little is known about its intracellular mechanism of action. Here we show that the stimulatory effect of caffeine on motor activity in mice was greatly reduced following genetic deletion of DARPP-32 (dopamine- and cyclic AMP-regulated phosphoprotein of relative molecular mass 32, 000). Results virtually identical to those seen with caffeine were obtained with the selective A2A antagonist SCH 58261. The depressant effect of the A2A receptor agonist, CGS 21680, on motor activity was also greatly attenuated in DARPP-32 knockout mice. In support of a role for DARPP-32 in the action of caffeine, we found that, in striata of intact mice, caffeine increased the state of phosphorylation of DARPP-32 at Thr 75. Caffeine increased Thr 75 phosphorylation through inhibition of PP-2A-catalysed dephosphorylation, rather than through stimulation of cyclin-dependent kinase 5 (Cdk5)-catalysed phosphorylation, of this residue. Together, these studies demonstrate the involvement of DARPP-32 and its phosphorylation/dephosphorylation in the stimulant action of caffeine.

Original languageEnglish (US)
Pages (from-to)774-778
Number of pages5
JournalNature
Volume418
Issue number6899
DOIs
StatePublished - Aug 15 2002

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    Lindskog, M., Svenningsson, P., Pozzi, L., Kim, Y., Flenberg, A. A., Bibb, J. A., Fredholm, B. B., Nairn, A. C., Greengard, P., & Fisone, G. (2002). Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine. Nature, 418(6899), 774-778. https://doi.org/10.1038/nature00817