Involvement of dectin-2 in ultraviolet radiation-induced tolerance

Yoshinori Aragane, Akira Maeda, Agatha Schwarz, Tadashi Tezuka, Kiyoshi Ariizumi, Thomas Schwarz

Research output: Contribution to journalArticle

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Abstract

Hapten sensitization through UV-exposed skin induces hapten-specific tolerance which can be adoptively transferred by injecting T cells into naive recipients. The exact phenotype of the regulatory T cells responsible for inhibiting the immune response and their mode of action remain largely unclear. Dectin-2 is a C-type lectin receptor expressed on APCs. It was postulated that dectin-2 interacts with its putative ligands on T cells and that the interaction may deliver costimulatory signals in naive T cells. Using a soluble fusion protein of dectin-2 (sDec2) which should inhibit this interaction, we studied the effect on contact hypersensitivity (CHS) and its modulation by UV radiation. Injection of sDec2 affected neither the induction nor the elicitation phase of CHS. In contrast, UV-induced inhibition of the CHS induction was prevented upon injection of sDec2. In addition, hapten-specific tolerance did not develop. Even more importantly, injection of sDec2 into tolerized mice rendered the recipients susceptible to the specific hapten, indicating that sDec2 can break established tolerance. FACS analysis of spleen and lymph node cells revealed a significantly increased portion of sDec2-binding T cells in UV-tolerized mice. Furthermore, transfer of UV-mediated suppression was lost upon depletion of the sDec2-positive T cells. Taken together, these data indicate that dectin-2 and its yet unidentified ligand may play a crucial role in the mediation of UV-induced immunosuppression. Moreover, sDec2-reactive T cells appear to represent the regulatory T cells responsible for mediating UV-induced tolerance.

Original languageEnglish (US)
Pages (from-to)3801-3807
Number of pages7
JournalJournal of Immunology
Volume171
Issue number7
StatePublished - Oct 1 2003

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Radiation Tolerance
Haptens
T-Lymphocytes
Contact Dermatitis
Regulatory T-Lymphocytes
Injections
Ligands
C-Type Lectins
Cell Communication
Immunosuppression
mouse dectin-2
Spleen
Lymph Nodes
Radiation
Phenotype
Skin
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Aragane, Y., Maeda, A., Schwarz, A., Tezuka, T., Ariizumi, K., & Schwarz, T. (2003). Involvement of dectin-2 in ultraviolet radiation-induced tolerance. Journal of Immunology, 171(7), 3801-3807.

Involvement of dectin-2 in ultraviolet radiation-induced tolerance. / Aragane, Yoshinori; Maeda, Akira; Schwarz, Agatha; Tezuka, Tadashi; Ariizumi, Kiyoshi; Schwarz, Thomas.

In: Journal of Immunology, Vol. 171, No. 7, 01.10.2003, p. 3801-3807.

Research output: Contribution to journalArticle

Aragane, Y, Maeda, A, Schwarz, A, Tezuka, T, Ariizumi, K & Schwarz, T 2003, 'Involvement of dectin-2 in ultraviolet radiation-induced tolerance', Journal of Immunology, vol. 171, no. 7, pp. 3801-3807.
Aragane Y, Maeda A, Schwarz A, Tezuka T, Ariizumi K, Schwarz T. Involvement of dectin-2 in ultraviolet radiation-induced tolerance. Journal of Immunology. 2003 Oct 1;171(7):3801-3807.
Aragane, Yoshinori ; Maeda, Akira ; Schwarz, Agatha ; Tezuka, Tadashi ; Ariizumi, Kiyoshi ; Schwarz, Thomas. / Involvement of dectin-2 in ultraviolet radiation-induced tolerance. In: Journal of Immunology. 2003 ; Vol. 171, No. 7. pp. 3801-3807.
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