Involvement of eicosanoids in release of oxytocin and vasopressin from the neural lobe of the rat pituitary

A. Negro-Vilar, G. D. Snyder, J R Falck, S. Manna, N. Chacos, J. Capdevila

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Arachidonic acid (AA) is oxidized via three pathways which result in several series of distinct metabolites. Cyclooxygenase produces prostaglandins (PGs), prostacyclins, and thromboxanes. Lipoxygenase produces hydroperoxy/hydroxyeicosatetraenoic acids (HPETE/HETEs) and leukotrienes. Epoxygenase, a recently uncovered pathway, results in epoxyeicosatrienoic acids (EETs). Based on reverse phase HPLC product analysis, this study establishes that all three pathways of AA metabolism are present in microsomal incubates of the neural lobe of the pituitary gland. Addition of PGE2 to incubated fragments of neural lobes of the rat pituitary stimulates secretion of both arginine vasopressin (AVP) and oxytocin in vitro. Inclusion of 5-HETE and 12-HETE in the incubation medium stimulates marginal release of AVP and oxytocin by 12-HETE only. The magnitude of AVP and oxytocin secretion stimulated by the epoxygenase metabolites 8,9-, 11,12-, and 14,15-EET is equal to that caused by PGE2. Maximal stimulation of secretion (3- to 4-fold) requires an EET concentration 10-15 times greater than that of PGE2. In contrast, 5,6-EET is inactive. These data suggest that oxygenated products of AA play a role in AVP and oxytocin secretion. Although PGs appear to be the dominant arachidonate metabolites involved in the release of AVP and oxytocin, the EETs probably have a contributing role.

Original languageEnglish (US)
Pages (from-to)2663-2668
Number of pages6
JournalEndocrinology
Volume116
Issue number6
StatePublished - 1985

Fingerprint

Vasotocin
Posterior Pituitary Gland
Eicosanoids
Arginine Vasopressin
Oxytocin
Vasopressins
Dinoprostone
Arachidonic Acid
Hydroxyeicosatetraenoic Acids
Prostaglandins I
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Leukotriene B4
Lipoxygenase
Leukotrienes
Thromboxanes
Pituitary Gland
Prostaglandin-Endoperoxide Synthases
Prostaglandins
High Pressure Liquid Chromatography
Acids

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Negro-Vilar, A., Snyder, G. D., Falck, J. R., Manna, S., Chacos, N., & Capdevila, J. (1985). Involvement of eicosanoids in release of oxytocin and vasopressin from the neural lobe of the rat pituitary. Endocrinology, 116(6), 2663-2668.

Involvement of eicosanoids in release of oxytocin and vasopressin from the neural lobe of the rat pituitary. / Negro-Vilar, A.; Snyder, G. D.; Falck, J R; Manna, S.; Chacos, N.; Capdevila, J.

In: Endocrinology, Vol. 116, No. 6, 1985, p. 2663-2668.

Research output: Contribution to journalArticle

Negro-Vilar, A, Snyder, GD, Falck, JR, Manna, S, Chacos, N & Capdevila, J 1985, 'Involvement of eicosanoids in release of oxytocin and vasopressin from the neural lobe of the rat pituitary', Endocrinology, vol. 116, no. 6, pp. 2663-2668.
Negro-Vilar, A. ; Snyder, G. D. ; Falck, J R ; Manna, S. ; Chacos, N. ; Capdevila, J. / Involvement of eicosanoids in release of oxytocin and vasopressin from the neural lobe of the rat pituitary. In: Endocrinology. 1985 ; Vol. 116, No. 6. pp. 2663-2668.
@article{d6efff4d07054ac4ae0d1a6ab965dfdd,
title = "Involvement of eicosanoids in release of oxytocin and vasopressin from the neural lobe of the rat pituitary",
abstract = "Arachidonic acid (AA) is oxidized via three pathways which result in several series of distinct metabolites. Cyclooxygenase produces prostaglandins (PGs), prostacyclins, and thromboxanes. Lipoxygenase produces hydroperoxy/hydroxyeicosatetraenoic acids (HPETE/HETEs) and leukotrienes. Epoxygenase, a recently uncovered pathway, results in epoxyeicosatrienoic acids (EETs). Based on reverse phase HPLC product analysis, this study establishes that all three pathways of AA metabolism are present in microsomal incubates of the neural lobe of the pituitary gland. Addition of PGE2 to incubated fragments of neural lobes of the rat pituitary stimulates secretion of both arginine vasopressin (AVP) and oxytocin in vitro. Inclusion of 5-HETE and 12-HETE in the incubation medium stimulates marginal release of AVP and oxytocin by 12-HETE only. The magnitude of AVP and oxytocin secretion stimulated by the epoxygenase metabolites 8,9-, 11,12-, and 14,15-EET is equal to that caused by PGE2. Maximal stimulation of secretion (3- to 4-fold) requires an EET concentration 10-15 times greater than that of PGE2. In contrast, 5,6-EET is inactive. These data suggest that oxygenated products of AA play a role in AVP and oxytocin secretion. Although PGs appear to be the dominant arachidonate metabolites involved in the release of AVP and oxytocin, the EETs probably have a contributing role.",
author = "A. Negro-Vilar and Snyder, {G. D.} and Falck, {J R} and S. Manna and N. Chacos and J. Capdevila",
year = "1985",
language = "English (US)",
volume = "116",
pages = "2663--2668",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "6",

}

TY - JOUR

T1 - Involvement of eicosanoids in release of oxytocin and vasopressin from the neural lobe of the rat pituitary

AU - Negro-Vilar, A.

AU - Snyder, G. D.

AU - Falck, J R

AU - Manna, S.

AU - Chacos, N.

AU - Capdevila, J.

PY - 1985

Y1 - 1985

N2 - Arachidonic acid (AA) is oxidized via three pathways which result in several series of distinct metabolites. Cyclooxygenase produces prostaglandins (PGs), prostacyclins, and thromboxanes. Lipoxygenase produces hydroperoxy/hydroxyeicosatetraenoic acids (HPETE/HETEs) and leukotrienes. Epoxygenase, a recently uncovered pathway, results in epoxyeicosatrienoic acids (EETs). Based on reverse phase HPLC product analysis, this study establishes that all three pathways of AA metabolism are present in microsomal incubates of the neural lobe of the pituitary gland. Addition of PGE2 to incubated fragments of neural lobes of the rat pituitary stimulates secretion of both arginine vasopressin (AVP) and oxytocin in vitro. Inclusion of 5-HETE and 12-HETE in the incubation medium stimulates marginal release of AVP and oxytocin by 12-HETE only. The magnitude of AVP and oxytocin secretion stimulated by the epoxygenase metabolites 8,9-, 11,12-, and 14,15-EET is equal to that caused by PGE2. Maximal stimulation of secretion (3- to 4-fold) requires an EET concentration 10-15 times greater than that of PGE2. In contrast, 5,6-EET is inactive. These data suggest that oxygenated products of AA play a role in AVP and oxytocin secretion. Although PGs appear to be the dominant arachidonate metabolites involved in the release of AVP and oxytocin, the EETs probably have a contributing role.

AB - Arachidonic acid (AA) is oxidized via three pathways which result in several series of distinct metabolites. Cyclooxygenase produces prostaglandins (PGs), prostacyclins, and thromboxanes. Lipoxygenase produces hydroperoxy/hydroxyeicosatetraenoic acids (HPETE/HETEs) and leukotrienes. Epoxygenase, a recently uncovered pathway, results in epoxyeicosatrienoic acids (EETs). Based on reverse phase HPLC product analysis, this study establishes that all three pathways of AA metabolism are present in microsomal incubates of the neural lobe of the pituitary gland. Addition of PGE2 to incubated fragments of neural lobes of the rat pituitary stimulates secretion of both arginine vasopressin (AVP) and oxytocin in vitro. Inclusion of 5-HETE and 12-HETE in the incubation medium stimulates marginal release of AVP and oxytocin by 12-HETE only. The magnitude of AVP and oxytocin secretion stimulated by the epoxygenase metabolites 8,9-, 11,12-, and 14,15-EET is equal to that caused by PGE2. Maximal stimulation of secretion (3- to 4-fold) requires an EET concentration 10-15 times greater than that of PGE2. In contrast, 5,6-EET is inactive. These data suggest that oxygenated products of AA play a role in AVP and oxytocin secretion. Although PGs appear to be the dominant arachidonate metabolites involved in the release of AVP and oxytocin, the EETs probably have a contributing role.

UR - http://www.scopus.com/inward/record.url?scp=0021861540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021861540&partnerID=8YFLogxK

M3 - Article

C2 - 3922746

AN - SCOPUS:0021861540

VL - 116

SP - 2663

EP - 2668

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 6

ER -