Involvement of endothelial CD44 during in vivo angiogenesis

Gaoyuan Cao, Rashmin C. Savani, Melane Fehrenbach, Chris Lyons, Lin Zhang, George Coukos, Horace M. DeLisser

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

CD44, a cell-surface receptor for hyaluronan, has been implicated in endothelial cell functions, but its role in the formation of blood vessels in vivo has not been established. In CD44-null mice, vascularization of Matrigel implants and tumor and wound angiogenesis were inhibited. Leukocyte accumulation during tumor growth and wound healing in wild-type and CD44-null mice were comparable, and reconstitution of CD44-null mice with wild-type bone marrow did not restore the wild-type phenotype, suggesting that impairments in angiogenesis hi CD44-deficient mice are due to the loss of endothelial CD44. Although the cell proliferation, survival, and wound-induced migration of CD44-null endothelial cells were intact, these cells were impaired in their in vitro ability to form tubes. Nascent vessels in Matrigel implants from CD44-null mice demonstrated irregular luminal surfaces characterized by retracted cells and thinned endothelia. Further, an anti-CD44 antibody that disrupted in vitro tube formation induced hemorrhage around Matrigel implants, suggesting that antagonism of endothelial CD44 undermined the integrity of the endothelium of nascent vessels. These data establish a role for CD44 during in vivo angiogenesis and suggest that CD44 may contribute to the organization and/or stability of developing endothelial tubular networks.

Original languageEnglish (US)
Pages (from-to)325-336
Number of pages12
JournalAmerican Journal of Pathology
Volume169
Issue number1
DOIs
StatePublished - Jul 2006

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Endothelium
Endothelial Cells
Null Lymphocytes
Wounds and Injuries
Cell Surface Receptors
Hyaluronic Acid
Wound Healing
Blood Vessels
Anti-Idiotypic Antibodies
Neoplasms
Cell Survival
Leukocytes
Bone Marrow
Cell Proliferation
Hemorrhage
Phenotype
Growth
matrigel
In Vitro Techniques

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Cao, G., Savani, R. C., Fehrenbach, M., Lyons, C., Zhang, L., Coukos, G., & DeLisser, H. M. (2006). Involvement of endothelial CD44 during in vivo angiogenesis. American Journal of Pathology, 169(1), 325-336. https://doi.org/10.2353/ajpath.2006.060206

Involvement of endothelial CD44 during in vivo angiogenesis. / Cao, Gaoyuan; Savani, Rashmin C.; Fehrenbach, Melane; Lyons, Chris; Zhang, Lin; Coukos, George; DeLisser, Horace M.

In: American Journal of Pathology, Vol. 169, No. 1, 07.2006, p. 325-336.

Research output: Contribution to journalArticle

Cao, G, Savani, RC, Fehrenbach, M, Lyons, C, Zhang, L, Coukos, G & DeLisser, HM 2006, 'Involvement of endothelial CD44 during in vivo angiogenesis', American Journal of Pathology, vol. 169, no. 1, pp. 325-336. https://doi.org/10.2353/ajpath.2006.060206
Cao, Gaoyuan ; Savani, Rashmin C. ; Fehrenbach, Melane ; Lyons, Chris ; Zhang, Lin ; Coukos, George ; DeLisser, Horace M. / Involvement of endothelial CD44 during in vivo angiogenesis. In: American Journal of Pathology. 2006 ; Vol. 169, No. 1. pp. 325-336.
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