Involvement of Matrin 3 and SFPQ/NONO in the DNA damage response

Maayan Salton, Yaniv Lerenthal, Shih Ya Wang, David J. Chen, Yosef Shiloh

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

The DNA damage response (DDR) is a complex signaling network that is induced by DNA lesions and vigorously activated by double strand breaks (DSBs). the DSB response is mobilized by the nuclear protein kinase AtM, which phosphorylates key players in its various branches. SFpQ (pSF) and NoNo (p54) are nuclear proteins that interact with each other and have diverse roles in nucleic acids metabolism. the SFpQ/NoNo heterodimer was previously found to enhance DNA strand break rejoining in vitro. our attention was drawn to these two proteins as they interact with the nuclear matrix protein Matrin 3 (MAtR3), which we found to be a novel AtM target. We asked whether SFpQ and NoNo too are involved in the DSB response. proteins that function at the early phase of this response are often recruited to the damaged sites. We observed rapid recruitment of SFpQ/NoNo to sites of DNA damage induced by laser microbeam. In MAtR3 knockdown cells SFpQ/NoNo retention at DNA damage sites was prolonged. SFpQ and MAtR3 depletion led to abnormal accumulation of cells at the S-phase of the cell cycle following treatment with the radiomimetic chemical neocarzinostatin. Notably, proteins involved in DSB repair via nonhomologous end-joining co-immunoprecipitated with NoNo; SFpQ depletion delayed DSB repair. Collectively the data suggest that SFpQ, NoNo and MAtR3 are involved in the early stage of the DSB response, setting the scene for DSB repair.

Original languageEnglish (US)
Pages (from-to)1568-1576
Number of pages9
JournalCell Cycle
Volume9
Issue number8
StatePublished - Apr 15 2010

Fingerprint

DNA Damage
Nuclear Proteins
Zinostatin
Nuclear Matrix-Associated Proteins
Proteins
DNA Breaks
S Phase
Protein Kinases
Nucleic Acids
Cell Cycle
Lasers
DNA
In Vitro Techniques

Keywords

  • ATM
  • Cell cycle checkpoint
  • DNA damage response
  • Matrin 3
  • NONO/p54
  • SFPQ/PSF

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

Salton, M., Lerenthal, Y., Wang, S. Y., Chen, D. J., & Shiloh, Y. (2010). Involvement of Matrin 3 and SFPQ/NONO in the DNA damage response. Cell Cycle, 9(8), 1568-1576.

Involvement of Matrin 3 and SFPQ/NONO in the DNA damage response. / Salton, Maayan; Lerenthal, Yaniv; Wang, Shih Ya; Chen, David J.; Shiloh, Yosef.

In: Cell Cycle, Vol. 9, No. 8, 15.04.2010, p. 1568-1576.

Research output: Contribution to journalArticle

Salton, M, Lerenthal, Y, Wang, SY, Chen, DJ & Shiloh, Y 2010, 'Involvement of Matrin 3 and SFPQ/NONO in the DNA damage response', Cell Cycle, vol. 9, no. 8, pp. 1568-1576.
Salton M, Lerenthal Y, Wang SY, Chen DJ, Shiloh Y. Involvement of Matrin 3 and SFPQ/NONO in the DNA damage response. Cell Cycle. 2010 Apr 15;9(8):1568-1576.
Salton, Maayan ; Lerenthal, Yaniv ; Wang, Shih Ya ; Chen, David J. ; Shiloh, Yosef. / Involvement of Matrin 3 and SFPQ/NONO in the DNA damage response. In: Cell Cycle. 2010 ; Vol. 9, No. 8. pp. 1568-1576.
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