Involvement of Rh blood group polypeptides in the maintenance of aminophospholipid asymmetry

Alan J. Schroit, Christian Bloy, Jerome Connor, Jean Pierre Cartron

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, 125I-azido-PS, and with an inhibitor of PS transport, 125I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS.

Original languageEnglish (US)
Pages (from-to)10303-10306
Number of pages4
JournalBiochemistry®
Volume29
Issue number45
StatePublished - 1990

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Phosphatidylserines
Blood Group Antigens
Maintenance
Phospholipid Transfer Proteins
Peptides
Monoclonal antibodies
Physical properties
Erythrocytes
Monoclonal Antibodies
Membranes
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Involvement of Rh blood group polypeptides in the maintenance of aminophospholipid asymmetry. / Schroit, Alan J.; Bloy, Christian; Connor, Jerome; Cartron, Jean Pierre.

In: Biochemistry®, Vol. 29, No. 45, 1990, p. 10303-10306.

Research output: Contribution to journalArticle

Schroit, AJ, Bloy, C, Connor, J & Cartron, JP 1990, 'Involvement of Rh blood group polypeptides in the maintenance of aminophospholipid asymmetry', Biochemistry®, vol. 29, no. 45, pp. 10303-10306.
Schroit, Alan J. ; Bloy, Christian ; Connor, Jerome ; Cartron, Jean Pierre. / Involvement of Rh blood group polypeptides in the maintenance of aminophospholipid asymmetry. In: Biochemistry®. 1990 ; Vol. 29, No. 45. pp. 10303-10306.
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