Involvement of Rh Blood Group Polypeptides in the Maintenance of Aminophospholipid Asymmetry

Alan J. Schroit, Christian Bloy, Jerome Connor, Jean Pierre Cartron

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Abstract

The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, 125I-azido-PS, and with an inhibitor of PS transport, 125I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS.

Original languageEnglish (US)
Pages (from-to)10303-10306
Number of pages4
JournalBiochemistry
Volume29
Issue number45
DOIs
StatePublished - Nov 1 1990

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ASJC Scopus subject areas

  • Biochemistry

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