Iron homeostasis regulates the activity of the microRNA pathway through poly(C)-binding protein 2

Yujing Li, Li Lin, Zigang Li, Xuecheng Ye, Ke Xiong, Baikuntha Aryal, Zihui Xu, Zain Paroo, Qinghua Liu, Chuan He, Peng Jin

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

MicroRNAs (miRNAs) control gene expression by promoting degradation or repressing translation of target mRNAs. The components of the miRNA pathway are subject to diverse modifications that can modulate the abundance and function of miRNAs. Iron is essential for fundamental metabolic processes, and its homeostasis is tightly regulated. Here we identified iron chelators as a class of activator of the miRNA pathway that could promote the processing of miRNA precursors. We show that cytosolic iron could regulate the activity of the miRNA pathway through poly(C)-binding protein 2 (PCBP2). PCBP2 is associated with Dicer and promotes the processing of miRNA precursors. Cytosolic iron could modulate the association between PCBP2 and Dicer, as well as the multimerization of PCBP2 and its ability to bind to miRNA precursors, which can alter the processing of miRNA precursors. Our findings reveal a role of iron homeostasis in the regulation of miRNA biogenesis.

Original languageEnglish (US)
Pages (from-to)895-904
Number of pages10
JournalCell Metabolism
Volume15
Issue number6
DOIs
StatePublished - Jun 6 2012

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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    Li, Y., Lin, L., Li, Z., Ye, X., Xiong, K., Aryal, B., Xu, Z., Paroo, Z., Liu, Q., He, C., & Jin, P. (2012). Iron homeostasis regulates the activity of the microRNA pathway through poly(C)-binding protein 2. Cell Metabolism, 15(6), 895-904. https://doi.org/10.1016/j.cmet.2012.04.021