Is cytomegalovirus associated with renal disease in AIDS patients?

T. Nadasdy, K. W. Miller, L. D. Johnson, O. Hanson-Painton, L. E. DeBault, D. K. Burns, E. Hawkins, F. G. Silva

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Abstract

Although it has been suggested that cytomegalovirus (CMV) infection of the kidney might facilitate the development of human immunodeficiency virus-associated nephropathy (HIVAN) or other morphologic renal changes in patients with AIDS, no systematic study has been performed on kidneys from AIDS patients. We examined 75 autopsy kidneys, two renal biopsy specimens, and a nephrectomy specimen from 78 HIV-infected patients (five with HIVAN) for the presence of CMV. Immunocytochemistry (ICC) utilizing a monoclonal antibody against the late antigen of CMV and in situ hybridization (ISH) with a biotinylated DNA probe for CMV sequences were used. The detection system for both ICC and ISH was streptavidin-conjugated alkaline phosphatase with Fast Red TR chromogen. CMV was detected in only 10 of the 78 kidneys examined (12.8%): eight by both methods, one by ISH only, and another by ICC only. All 10 positive kidneys were obtained from autopsies of patients with AIDS. The average number of positive cells (in approximately 15 x 10 mm sections) was 22 with ICC and 10 with ISH. Glomerular intracapillary cells (possibly endothelial cells) were the most commonly stained, followed by positive cells in the interstitium and peritubular capillaries. Relatively few tubular epithelial cells were stained. The majority of positive cells by either ICC or ISH did not show nuclear or cytoplasmic inclusions; however, only two of the 10 positive kidneys did not contain cells with typical Cowdry type-A intranuclear CMV inclusions. The most frequent pathologic finding in the kidneys positive for CMV by either ICC or ISH was acute tubular necrosis (in six of 10, 60%).(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)277-282
Number of pages6
JournalModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Volume5
Issue number3
StatePublished - May 1992

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Cytomegalovirus
Acquired Immunodeficiency Syndrome
Kidney
In Situ Hybridization
Immunohistochemistry
AIDS-Associated Nephropathy
Intranuclear Inclusion Bodies
Autopsy
Streptavidin
Inclusion Bodies
DNA Probes
Cytomegalovirus Infections
Nephrectomy
Alkaline Phosphatase
Necrosis
Endothelial Cells
Cell Count
Epithelial Cells
Monoclonal Antibodies
HIV

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Nadasdy, T., Miller, K. W., Johnson, L. D., Hanson-Painton, O., DeBault, L. E., Burns, D. K., ... Silva, F. G. (1992). Is cytomegalovirus associated with renal disease in AIDS patients? Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 5(3), 277-282.

Is cytomegalovirus associated with renal disease in AIDS patients? / Nadasdy, T.; Miller, K. W.; Johnson, L. D.; Hanson-Painton, O.; DeBault, L. E.; Burns, D. K.; Hawkins, E.; Silva, F. G.

In: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 5, No. 3, 05.1992, p. 277-282.

Research output: Contribution to journalArticle

Nadasdy, T, Miller, KW, Johnson, LD, Hanson-Painton, O, DeBault, LE, Burns, DK, Hawkins, E & Silva, FG 1992, 'Is cytomegalovirus associated with renal disease in AIDS patients?', Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, vol. 5, no. 3, pp. 277-282.
Nadasdy, T. ; Miller, K. W. ; Johnson, L. D. ; Hanson-Painton, O. ; DeBault, L. E. ; Burns, D. K. ; Hawkins, E. ; Silva, F. G. / Is cytomegalovirus associated with renal disease in AIDS patients?. In: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 1992 ; Vol. 5, No. 3. pp. 277-282.
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