Ischemia-reperfusion induces G-CSF gene expression by renal medullary thick ascending limb cells in vivo and in vitro

Ying Zhang, Vanessa K. Woodward, John M. Shelton, James A. Richardson, Xin J. Zhou, Daniel Link, Mariusz L. Kielar, D. Rohan Jeyarajah, Christopher Y. Lu

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Ischemic acute renal failure involves not only the kidney but also extrarenal organs such as the bone marrow that produces inflammatory cells. By ELISA and RNase protection assays, we now show that renal ischemia-reperfusion increases serum concentrations of granulocyte macrophage colony-stimulating factor (G-CSF) protein and increases both G-CSF mRNA and protein in the ischemic kidney. In situ hybridization localized the increased G-CSF mRNA to tubule cells, including medullary thick ascending limb cells (mTAL), in the outer medulla. We also show that mTAL produce G-CSF protein and increase G-CSF mRNA after stimulation by reactive oxygen species in vitro. The production of G-CSF by the kidney after ischemia-reperfusion provides a means of communication from the injured kidney to the bone marrow. This supports the known inflammatory response to ischemia.

Original languageEnglish (US)
Pages (from-to)F1193-F1201
JournalAmerican Journal of Physiology - Renal Physiology
Volume286
Issue number6 55-6
DOIs
StatePublished - Jun 1 2004

Keywords

  • Acute renal failure
  • Granulocyte colony-stimulating factor
  • Ischemia
  • Medullary thick ascending limb tubules
  • Reactive oxygen species

ASJC Scopus subject areas

  • Physiology
  • Urology

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