Isolation and analysis of a mammalian temperature-sensitive mutant defective in G2 functions

Chieko Mineo, Yasufumi Murakami, Yukio Ishimi, Fumio Hanaoka, Masa Atsu Yamada

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Abstract

A temperature-sensitive (ts) mutant, designated tsFT210, was isolated from a mouse mammary carcinoma cell line, FM3A. The tsFT210 cells grew normally at 33 °C (permissive temperature), but more than 80% of the cells were arrested at the G2 phase at 39 °C (nonpermissive temperature) as revealed by flow-microfluorimetric analysis. DNA replication and synthesis of other macromolecules by this mutant seemed to be normal at 39 °C for at least 10 h. However, in this mutant, hyperphosphorylation of H1 histone from the G2 to M phase, which occurs in the normal cell cycle, could not be detected at the non-permissive temperature. This suggests that a gene product which is temperature-sensitive in tsFT210 cells is necessary for hyperphosphorylation of H1 histone and that this gene product may be related to chromosome condensation.

Original languageEnglish (US)
Pages (from-to)53-62
Number of pages10
JournalExperimental Cell Research
Volume167
Issue number1
DOIs
StatePublished - Nov 1986

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ASJC Scopus subject areas

  • Cell Biology

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