Isolation and analysis of a mammalian temperature-sensitive mutant defective in G2 functions

Chieko Mineo, Yasufumi Murakami, Yukio Ishimi, Fumio Hanaoka, Masa Atsu Yamada

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Abstract

A temperature-sensitive (ts) mutant, designated tsFT210, was isolated from a mouse mammary carcinoma cell line, FM3A. The tsFT210 cells grew normally at 33 °C (permissive temperature), but more than 80% of the cells were arrested at the G2 phase at 39 °C (nonpermissive temperature) as revealed by flow-microfluorimetric analysis. DNA replication and synthesis of other macromolecules by this mutant seemed to be normal at 39 °C for at least 10 h. However, in this mutant, hyperphosphorylation of H1 histone from the G2 to M phase, which occurs in the normal cell cycle, could not be detected at the non-permissive temperature. This suggests that a gene product which is temperature-sensitive in tsFT210 cells is necessary for hyperphosphorylation of H1 histone and that this gene product may be related to chromosome condensation.

Original languageEnglish (US)
Pages (from-to)53-62
Number of pages10
JournalExperimental Cell Research
Volume167
Issue number1
DOIs
StatePublished - 1986

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Temperature
Histones
G2 Phase
DNA Replication
Cell Division
Genes
Cell Cycle
Chromosomes
Breast Neoplasms
Cell Line

ASJC Scopus subject areas

  • Cell Biology

Cite this

Isolation and analysis of a mammalian temperature-sensitive mutant defective in G2 functions. / Mineo, Chieko; Murakami, Yasufumi; Ishimi, Yukio; Hanaoka, Fumio; Yamada, Masa Atsu.

In: Experimental Cell Research, Vol. 167, No. 1, 1986, p. 53-62.

Research output: Contribution to journalArticle

Mineo, Chieko ; Murakami, Yasufumi ; Ishimi, Yukio ; Hanaoka, Fumio ; Yamada, Masa Atsu. / Isolation and analysis of a mammalian temperature-sensitive mutant defective in G2 functions. In: Experimental Cell Research. 1986 ; Vol. 167, No. 1. pp. 53-62.
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