Isolation and analysis of discreet human prostate cellular populations

Douglas W. Strand, LaTayia Aaron, Gervaise Henry, Omar E. Franco, Simon W. Hayward

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.

Original languageEnglish (US)
JournalDifferentiation
DOIs
StateAccepted/In press - Oct 23 2015

Fingerprint

Prostate
Population
Transgenic Mice
Flow Cytometry
Homeostasis
Cell Culture Techniques
Phenotype
Research

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology
  • Cancer Research

Cite this

Isolation and analysis of discreet human prostate cellular populations. / Strand, Douglas W.; Aaron, LaTayia; Henry, Gervaise; Franco, Omar E.; Hayward, Simon W.

In: Differentiation, 23.10.2015.

Research output: Contribution to journalArticle

Strand, Douglas W. ; Aaron, LaTayia ; Henry, Gervaise ; Franco, Omar E. ; Hayward, Simon W. / Isolation and analysis of discreet human prostate cellular populations. In: Differentiation. 2015.
@article{483495e0f4ed43bb9e62d5dfeadc1a90,
title = "Isolation and analysis of discreet human prostate cellular populations",
abstract = "The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.",
author = "Strand, {Douglas W.} and LaTayia Aaron and Gervaise Henry and Franco, {Omar E.} and Hayward, {Simon W.}",
year = "2015",
month = "10",
day = "23",
doi = "10.1016/j.diff.2015.10.013",
language = "English (US)",
journal = "Differentiation",
issn = "0301-4681",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Isolation and analysis of discreet human prostate cellular populations

AU - Strand, Douglas W.

AU - Aaron, LaTayia

AU - Henry, Gervaise

AU - Franco, Omar E.

AU - Hayward, Simon W.

PY - 2015/10/23

Y1 - 2015/10/23

N2 - The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.

AB - The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.

UR - http://www.scopus.com/inward/record.url?scp=84953321046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84953321046&partnerID=8YFLogxK

U2 - 10.1016/j.diff.2015.10.013

DO - 10.1016/j.diff.2015.10.013

M3 - Article

C2 - 26546040

AN - SCOPUS:84953321046

JO - Differentiation

JF - Differentiation

SN - 0301-4681

ER -