Isolation and analysis of discreet human prostate cellular populations

Douglas W. Strand, LaTayia Aaron, Gervaise Henry, Omar E. Franco, Simon W. Hayward

Research output: Contribution to journalReview articlepeer-review

8 Scopus citations

Abstract

The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.

Original languageEnglish (US)
Pages (from-to)139-151
Number of pages13
JournalDifferentiation
Volume91
Issue number4-5
DOIs
StatePublished - Jun 1 2016

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

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