Isolation and analysis of discreet human prostate cellular populations

Douglas W. Strand; LaTayia Aaron; Gervaise Henry; Omar E. Franco; Simon W. Hayward

doi:10.1016/j.diff.2015.10.013

Isolation and analysis of discreet human prostate cellular populations

Douglas W. Strand, LaTayia Aaron, Gervaise Henry, Omar E. Franco, Simon W. Hayward

Research output: Contribution to journal › Review article › peer-review

13 Scopus citations

Abstract

The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.

Original language	English (US)
Pages (from-to)	139-151
Number of pages	13
Journal	Differentiation
Volume	91
Issue number	4-5
DOIs	https://doi.org/10.1016/j.diff.2015.10.013
State	Published - Jun 1 2016

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology
Cancer Research

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title = "Isolation and analysis of discreet human prostate cellular populations",

abstract = "The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.",

author = "Strand, {Douglas W.} and LaTayia Aaron and Gervaise Henry and Franco, {Omar E.} and Hayward, {Simon W.}",

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AU - Strand, Douglas W.

AU - Aaron, LaTayia

AU - Henry, Gervaise

AU - Franco, Omar E.

AU - Hayward, Simon W.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.

AB - The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.

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DO - 10.1016/j.diff.2015.10.013

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VL - 91

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JO - Differentiation

JF - Differentiation

IS - 4-5

ER -

Isolation and analysis of discreet human prostate cellular populations

Abstract

ASJC Scopus subject areas

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