Isolation and characterisation of a diverse family of Arabidopsis two and three-fingered C2H2 zinc finger protein genes and cDNAs

Ruth Meissner, Anthony J. Michael

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

In animal systems the C2H2 zinc finger protein (ZFP) gene family is the largest group of regulatory proteins and its members have a wide and important role in growth and development. It is likely that this family of ZFP transcription factors will also be important in plants. We have used a PCR approach employing highly degenerate oligonucleotide printers to isolate several Arabidopsis genomic and cDNA clones encoding potential ZFPs. In addition we have used the sequence information from these clones to identify two ESTs as members of this family. Five two-fingered and one three-fingered ZFPs have been identified. Outside of the zinc finger regions, there is considerable sequence diversity, including the sequence and length of the interfinger region; the expression pattern of each gene is different. This is the first report of the isolation of a three-fingered plant C2H2 ZFP gone and we discuss its possible evolutionary origin from two-fingered ZFP genes.

Original languageEnglish (US)
Pages (from-to)615-624
Number of pages10
JournalPlant Molecular Biology
Volume33
Issue number4
DOIs
StatePublished - Mar 1997

Fingerprint

zinc finger motif
Arabidopsis
Zinc
Complementary DNA
Zinc Fingers
Proteins
genes
proteins
Clone Cells
printers
clones
Expressed Sequence Tags
regulatory proteins
oligonucleotides
Growth and Development
Oligonucleotides
growth and development
Animals
Transcription Factors
transcription factors

Keywords

  • Arabidopsis thaliana
  • CH zinc finger protein
  • polymerase chain reaction
  • transcription factor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

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