Isolation and characterization of a glycine-extended form of calcitonin receptor-stimulating peptide-1: Another biologically active form of calcitonin receptor-stimulating peptide-1

Takeshi Katafuchi, Kazumasa Hamano, Katsuro Kikumoto, Naoto Minamino

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

In this study, we isolated a peptide eliciting a potent stimulatory effect on cAMP production in LLC-PK1 cells from acid extracts of porcine brain. By structural analysis, this peptide was determined to be a C-terminal glycine-extended form of calcitonin receptor-stimulating peptide-1 (CRSP-1-Gly). Synthetic CRSP-1-Gly enhanced the cAMP production in COS-7 cells expressing calcitonin (CT) receptor as strongly as CRSP-1. Measurement of immunoreactive (IR) CRSP-1-Gly by radioimmunoassay using the specific antisera against CRSP-1-Gly showed that a relatively high level (>1 pmol/g wet weight) of IR-CRSP-1-Gly was detected in the midbrain, hypothalamus, anterior and posterior lobes of pituitary, and thyroid gland, and the ratio of IR-CRSP-1-Gly to total IR-CRSP-1 varies from 0.02 to 0.35 in each tissue. These results suggest that CRSP-1-Gly is actually present in the tissues as one of major endogenous molecular forms of CRSP-1, and can regulate the cells expressing the CT receptor both in the central nervous system and peripheral tissues in a manner similar to that of CRSP-1. IR-CRSP-2 and IR-CRSP-3 are also present in the brain and other tissues, but their tissue concentrations are 33% on average and less than 3% that of total IR-CRSP-1, respectively.

Original languageEnglish (US)
Pages (from-to)2616-2623
Number of pages8
JournalPeptides
Volume26
Issue number12
DOIs
StatePublished - Dec 1 2005

Keywords

  • Calcitonin receptor
  • Calcitonin receptor-stimulating peptide
  • Glycine-extended form of calcitonin receptor-stimulating peptide
  • Isolation
  • Radioimmunoassay
  • cAMP

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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