Isolation and characterization of a molecular complex containing Thy 1 antigen from the surface of murine thymocytes and T cells

E. S. Vitetta, E. A. Boyse, J. W. Uhr

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Abstract

A complex containing the Thy-1 antigen has been isolated from the surface of murine thymocytes and T cells by cell surface radioiodination, lysis by freezing and thawing, and immunoprecipitation. The specificity of the immunoprecipitation was firmly established using both congenic and noncongenic anti Thy-1 sera and thymocytes from congenic and noncongenic mice. Unlike cell surface Ig and the TL and H 2 alloantigens, the antigenicity of Thy-1 was abolished by treatment with nonionic detergent. Thy-1 was localized exclusively in a complex of cell surface molecules which has a density lower than that of protein. Moreover, Thy-1 could be readily labeled by incubating thymocytes with [3H] galactose, but not 3H-labeled amino acids. These observations suggest that the antigenicity of Thy-1 may reside in a glycolipid.

Original languageEnglish (US)
Pages (from-to)446-453
Number of pages8
JournalEuropean Journal of Immunology
Volume3
Issue number7
StatePublished - 1973

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Thy-1 Antigens
Thymocytes
T-Lymphocytes
Immunoprecipitation
Congenic Mice
Isoantigens
Glycolipids
Galactose
Detergents
Freezing
Amino Acids
Serum
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Isolation and characterization of a molecular complex containing Thy 1 antigen from the surface of murine thymocytes and T cells. / Vitetta, E. S.; Boyse, E. A.; Uhr, J. W.

In: European Journal of Immunology, Vol. 3, No. 7, 1973, p. 446-453.

Research output: Contribution to journalArticle

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