TY - JOUR
T1 - Isolation and characterization of Na+/Ca2+ exchanger gene and splicing isoforrns in mice
AU - Wakimoto, Koji
AU - Kuro-O, Makoto
AU - Yanaka, Noriyuki
AU - Omori, Kenji
AU - Komuro, Issei
AU - Imai, Yuji
AU - Nabeshima, Yo Ichi
PY - 2000
Y1 - 2000
N2 - The Na+/Ca2+ exchanger gene NCXl is ubiquitously expressed in mammalian tissues, and encodes several isoforms through alternative RNA splicing. In this report, we describe the gene structure that gives rise to the multiple isoforms, and the tissue-specific expression of these isoforms in mice. The mouse NCXl gene contains a cluster of six exons (A, B, C, D, E, and F) which encode a variable region in the large intracellular loop of the protein, as previously reported in rabbits and humans. Using reverse tran-scription- polymerase chain reaction (RT-PCR), expression of the isoforms was examined in several tissues. We also identified a novel splice variant, which originate from exons A, C, D, and F. These findings provide new insights into the significance of the large repertoire of NCXl isoforms.
AB - The Na+/Ca2+ exchanger gene NCXl is ubiquitously expressed in mammalian tissues, and encodes several isoforms through alternative RNA splicing. In this report, we describe the gene structure that gives rise to the multiple isoforms, and the tissue-specific expression of these isoforms in mice. The mouse NCXl gene contains a cluster of six exons (A, B, C, D, E, and F) which encode a variable region in the large intracellular loop of the protein, as previously reported in rabbits and humans. Using reverse tran-scription- polymerase chain reaction (RT-PCR), expression of the isoforms was examined in several tissues. We also identified a novel splice variant, which originate from exons A, C, D, and F. These findings provide new insights into the significance of the large repertoire of NCXl isoforms.
KW - Na/Ca exchanger
KW - alternative splicing
KW - disribution
KW - gene
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U2 - 10.3109/10425170009033971
DO - 10.3109/10425170009033971
M3 - Article
C2 - 10902911
AN - SCOPUS:0033923992
SN - 1940-1736
VL - 11
SP - 75
EP - 81
JO - Mitochondrial DNA
JF - Mitochondrial DNA
IS - 1-2
ER -