TY - JOUR
T1 - Isolation and characterization of two new Drosophila protein kinase C genes, including one specifically expressed in photoreceptor cells
AU - Schaeffer, Eric
AU - Smith, Dean
AU - Mardon, Graeme
AU - Quinn, William
AU - Zuker, Charles
N1 - Funding Information:
This project was made possrble by Peter J Parker who gave us advice and bovrne PKC cDNA clones before publicabon. John Foster and Rob Jackson provided cGMP-dependent kinase clones for comparison. We thank Suzanne Seavello for her excellent help with chromosomal in SItu’s, Kwang-Wook Choi for insights into protein kinase C. and Norbert Perrimon for reagents and materials. E. S. is a Natronal lnslitutes of Health postdoctoral fellow, G. M. was a National Science Foundatron predoctoral fellow, and D. S. was supported by an NIH predoctoral grant. This work was supported by grants from the NIH and awards from the McKnight foundation to W. G. Q and C. Z and a Pew Scholars award to C. Z.
PY - 1989/5/5
Y1 - 1989/5/5
N2 - We have isolated and characterized two new protein kinase C (PKC) genes from D. melanogaster. One, dPKC98F, maps to chromosome region 98F and displays over 60% amino acid sequence identity with members of a recently described "PKC-related" subfamily in mammals. The other, dPKC53E(ey), maps to region 53E 4-7 on the second chromosome and lies within 50 kb of a PKC gene previously characterized (dPKC). While dPKC98F transcripts are expressed throughout development, expression of the two genes mapping at cytogenetic location 53E is primarily in adults. dPKC98F and the previously reported 53E gene are transcribed predominantly in brain tissue. In contrast, dPKC53E(ey) is transcribed only in photoreceptor cells. We will discuss the significance of this tissue-specific localization with regard to phototransduction.
AB - We have isolated and characterized two new protein kinase C (PKC) genes from D. melanogaster. One, dPKC98F, maps to chromosome region 98F and displays over 60% amino acid sequence identity with members of a recently described "PKC-related" subfamily in mammals. The other, dPKC53E(ey), maps to region 53E 4-7 on the second chromosome and lies within 50 kb of a PKC gene previously characterized (dPKC). While dPKC98F transcripts are expressed throughout development, expression of the two genes mapping at cytogenetic location 53E is primarily in adults. dPKC98F and the previously reported 53E gene are transcribed predominantly in brain tissue. In contrast, dPKC53E(ey) is transcribed only in photoreceptor cells. We will discuss the significance of this tissue-specific localization with regard to phototransduction.
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U2 - 10.1016/0092-8674(89)90915-X
DO - 10.1016/0092-8674(89)90915-X
M3 - Article
C2 - 2720775
AN - SCOPUS:0024961952
VL - 57
SP - 403
EP - 412
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -