@inbook{87c4e3ad75a84c3b9f0c2cc52959ac2b,
title = "Isolation and culture of oligodendrocyte precursor cells from prenatal and postnatal rodent brain",
abstract = "Oligodendrocytes, the myelinating cells in the vertebrate central nervous system, electrically insulate axons and facilitate rapid propagation of action potentials. The isolation and transplantation of oligodendroglial precursor cells (OPCs) could provide a powerful means to characterize their differentiation properties and potential and enhance myelin repair. Preparation and maintenance of rodent OPCs have been a challenge due to difficulties in obtaining a sufficient quantity. Here, we describe step-by-step and efficient methods to prepare highly enriched OPCs from rat and mice. Isolated OPCs can be induced to differentiate into oligodendrocytes and express most, if not all, of oligodendrocyte-associated genes in vivo. The ability to isolate and culture OPCs should facilitate studies on oligodendrocyte lineage progression and their utility in myelin repair after injury.",
keywords = "Immunopanning, Mechanical dissociation, Mixed glial culture, Mouse cortex, Neurosphere, OPC, Oligodendrocyte lineage, Oligodendrosphere, Rat cortex",
author = "Danyang He and Bradley Meyer and {Richard Lu}, Q.",
note = "Funding Information: Authors would like to thank Dr. Jianrong Li and Dr. Jonah Chan for sharing the rat and mouse OPC isolation protocols, respectively. This study was funded in part by grants from the US National Institutes of Health R01NS072427 and R01NS075243 to QRL and the National Multiple Sclerosis Society (NMSS-4727) to QRL Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media LLC.",
year = "2017",
doi = "10.1007/978-1-4939-7024-7_6",
language = "English (US)",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "95--109",
booktitle = "Neuromethods",
}