Isolation and culture of oligodendrocyte precursor cells from prenatal and postnatal rodent brain

Danyang He, Bradley Meyer, Q. Richard Lu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Oligodendrocytes, the myelinating cells in the vertebrate central nervous system, electrically insulate axons and facilitate rapid propagation of action potentials. The isolation and transplantation of oligodendroglial precursor cells (OPCs) could provide a powerful means to characterize their differentiation properties and potential and enhance myelin repair. Preparation and maintenance of rodent OPCs have been a challenge due to difficulties in obtaining a sufficient quantity. Here, we describe step-by-step and efficient methods to prepare highly enriched OPCs from rat and mice. Isolated OPCs can be induced to differentiate into oligodendrocytes and express most, if not all, of oligodendrocyte-associated genes in vivo. The ability to isolate and culture OPCs should facilitate studies on oligodendrocyte lineage progression and their utility in myelin repair after injury.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages95-109
Number of pages15
Volume126
DOIs
StatePublished - 2017

Publication series

NameNeuromethods
Volume126
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Immunopanning
  • Mechanical dissociation
  • Mixed glial culture
  • Mouse cortex
  • Neurosphere
  • Oligodendrocyte lineage
  • Oligodendrosphere
  • OPC
  • Rat cortex

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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  • Cite this

    He, D., Meyer, B., & Richard Lu, Q. (2017). Isolation and culture of oligodendrocyte precursor cells from prenatal and postnatal rodent brain. In Neuromethods (Vol. 126, pp. 95-109). (Neuromethods; Vol. 126). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7024-7_6