Isolation and culture of rat and mouse oligodendrocyte precursor cells

Ying Chen, Veerakumar Balasubramaniyan, Jie Peng, Edward C. Hurlock, Michelle Tallquist, Jianrong Li, Q. Richard Lu

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227 Scopus citations

Abstract

The ability to isolate oligodendroglial precursor cells (OPCs) provides a powerful means to characterize their differentiation, properties and potential for myelin repair. Although much knowledge is available for isolation of OPCs from the rat central nervous system, preparation and maintenance of mouse OPCs has been until recently a challenge owing to difficulties in obtaining a sufficient quantity of purified OPCs. Here, we describe protocols to prepare highly enriched rat OPCs and nearly homogenous mouse OPCs. The mouse method generates predominantly OPCs from cortical neural progenitor cells as clonal aggregates called "oligospheres" by taking advantage of molecular genetic tools. Isolated OPCs can be further differentiated into oligodendrocytes. Collectively, we describe simple and efficient methods for the preparation and in vitro maintenance of enriched OPCs from rats and mice. Isolation and culture of a large, homogenous population of rodent OPCs should significantly facilitate studies on OPC lineage progression and their utility in myelin repair after injury.

Original languageEnglish (US)
Pages (from-to)1044-1051
Number of pages8
JournalNature Protocols
Volume2
Issue number5
DOIs
StatePublished - May 1 2007

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Chen, Y., Balasubramaniyan, V., Peng, J., Hurlock, E. C., Tallquist, M., Li, J., & Lu, Q. R. (2007). Isolation and culture of rat and mouse oligodendrocyte precursor cells. Nature Protocols, 2(5), 1044-1051. https://doi.org/10.1038/nprot.2007.149