Isolation and partial characterization of virus-transformed cell lines representing the A, G and variant complementation groups of xeroderma pigmentosum

Dina P. Barbis, Roger A. Schultz, Errol C. Friedberg

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

We have established viral-transformed, apparently permanent (immortalized) cell lines from diploid fibroblasts representative of normal and xeroderma pigmentosum (XP) A, G and variant individuals. The XP-G and XP-variant cells represent complementation groups not previously available as permanent lines. All the new permanent cell lines exhibit SV40 T-antigen expression. They are also aneuploid and have growth characteristics typical of viral transformants. They have retained the phenotypes of UV sensitivity, reduced repair synthesis or defective 'postreplication repair' appropriate to the XP complementation group they represent. Additionally, the new cell lines are all transfectable with the selectable plasmid pRSVneo. The XP-G and XP-variant cell lines show enhanced transfection with UV-irradiated plasmid DNA; a phenomenon previously reported for normal immortalized cells and for immortalized cells from the A and F complementation groups of XP.

Original languageEnglish (US)
Pages (from-to)175-184
Number of pages10
JournalMutation Research DNA Repair Reports
Volume165
Issue number3
DOIs
StatePublished - May 1986

ASJC Scopus subject areas

  • Medicine(all)

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