Isolation and quantitation of adiponectin higher order complexes

Joseph M. Rutkowski, Philipp E. Scherer

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Adiponectin is a circulating bioactive hormone secreted by adipocytes as oligomers ranging in size from 90 kDa trimers and 180 kDa hexamers to larger high molecular weight oligomers that may reach 18- or 36-mers in size. While total circulating adiponectin levels correlate well with metabolic health, it is the relative distribution of adiponectin complexes that is most clinically relevant to glucose sensitivity and inflammation. High molecular weight adiponectin best mirrors insulin sensitivity, while trimeric adiponectin dominates with insulin resistance and adipose tissue inflammation. Experimental animal and in vitro models have also linked the relative fraction of high molecular weight adiponectin to its positive effects. Quantitating adiponectin size distribution thus provides a window into metabolic health and can serve as a surrogate marker for adipose tissue fitness. Here, we present a detailed protocol for isolating and quantitating adiponectin complexes in serum or plasma that has been extensively utilized for both human clinical samples and numerous animal models under various experimental conditions. Examples are presented of different adiponectin distributions and tips are provided for optimization using available equipment. Comparison of this rigorous approach to other available methods is also discussed. In total, this summary is a blueprint for the expanded quantitation and study of adiponectin complexes.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages243-259
Number of pages17
Volume537
ISBN (Print)9780124116191
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume537
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

Adiponectin
Molecular Weight
Molecular weight
Oligomers
Insulin Resistance
Adipose Tissue
Animals
Health
Insulin
Tissue
Inflammation
Blueprints
Adipocytes
Animal Models
Biomarkers
Hormones
Plasmas
Glucose
Equipment and Supplies

Keywords

  • Adipokine
  • Adiponectin
  • Complex distribution
  • Gel fractionation
  • HMW
  • LMW
  • Oligomer
  • Trimer

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Rutkowski, J. M., & Scherer, P. E. (2014). Isolation and quantitation of adiponectin higher order complexes. In Methods in Enzymology (Vol. 537, pp. 243-259). (Methods in Enzymology; Vol. 537). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-411619-1.00013-6

Isolation and quantitation of adiponectin higher order complexes. / Rutkowski, Joseph M.; Scherer, Philipp E.

Methods in Enzymology. Vol. 537 Academic Press Inc., 2014. p. 243-259 (Methods in Enzymology; Vol. 537).

Research output: Chapter in Book/Report/Conference proceedingChapter

Rutkowski, JM & Scherer, PE 2014, Isolation and quantitation of adiponectin higher order complexes. in Methods in Enzymology. vol. 537, Methods in Enzymology, vol. 537, Academic Press Inc., pp. 243-259. https://doi.org/10.1016/B978-0-12-411619-1.00013-6
Rutkowski JM, Scherer PE. Isolation and quantitation of adiponectin higher order complexes. In Methods in Enzymology. Vol. 537. Academic Press Inc. 2014. p. 243-259. (Methods in Enzymology). https://doi.org/10.1016/B978-0-12-411619-1.00013-6
Rutkowski, Joseph M. ; Scherer, Philipp E. / Isolation and quantitation of adiponectin higher order complexes. Methods in Enzymology. Vol. 537 Academic Press Inc., 2014. pp. 243-259 (Methods in Enzymology).
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